The effects of parathyroid hormone on the calcification ability of cultured human dental papilla mesenchymal cell
Abstract：AIM: Observation of the effects of parathyroid hormone on the calcification ability of cultured human dental papilla mesenchymal cell (DPMC). METHODS: Two groups of the fourth passage human DPMC were cultured for 35days and observed by transmission electron microscope (TEM), scanning electron microscope (SEM) and hght microscope (LM). The control group was cultured in DMEM medium supplemented with 15% FCS, 50mg/L ascorbic acid and 2mmol/L 3 - glycerophosphate. The PTH group was cultured in the medium of control group containing 33.3umol/L PTH. RESULTS:The cell presented mineralized nodules as well as matrix vesicles and had the same ultrastructure with odontoblasts under TEM. The addition of PTH in the medium caused not only a significant increase in the number of nodules but also the formation of many matrix vesicles. CONCLUSION: The results suggest that cultured human DMPC have the calcification ability which may be related to the matrix vesicles and PTH enhances the calcification ability of cultured human DPMC.
Keywords：dental papillamesenchymalparathyroid hormonecalcification
Publication Date：2000-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 314-317 )