Immunohistochemical localization of Calbindin- D28K in human teeth
Abstract：AIM: Investigation of the role of Calbindin- D28K(CaB) in the reparative process after pulp injury.METHODS: Immunohistochemical staining was adopted for studying the expression of CaB in healthy and carious teeth, and the results were analyzed by image analysis. RESULTS: CaB was detected in odontoblast layer, predenfin, vascular epitheliun cells, nerve fibers of both healthy and carious teeth. Some pulp cells fiom the teeth with intermediate or deep caries expressed a small amount of CaB. In the teeth with intermediate caries, the odontoblastic process, the cytoplasm, as well as some of nuclei stained positive strongly while the amount of odontoblasts increased. In the teeth with deep caries, predentin was thinner than that of the intermediate caries and stained positive, odontoblasts exhibited vacuolar degeneration. And odontoblast - like cells, which exhibited flat and aligned mono- layer, were observed and stained positive. By image analysis, the amount of of CaB in the cytoplasm of odontoblasts in the teeth with intermediate and deep caries was higher than that of the normal teeth. As to the content of CaB in vascular epithelium, there was no difference among three groups. CONCLUSION:CaB plays a significant role in differentiation of pulp cell to odontoblast, as well as formation of reparative and reactive dentin.
Keywords：Calbindin-D28Kdental pulpcariesimmunohistochemicalimage analysis system
Publication Date：2000-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 310-313 )