An investigation to the secretion mode of Hepatocyte Growth Factor/Scatter Factor by gingival fibroblasts in vitro
WU Zhi-qiang
LING Li
Thomas DW
Abstract：AIM:To investigate the secretion mode of hepatocyte growth factor/scatter factor(HGF/SF) by gingivalfibroblasts in vitro. MEFHODS: Gingival fibroblasts were cultured, and the fibroblast populated collagen lattices (FPCLs) were farbricatad and divided into 3 groups. The diameter of the FPCL was measured from day 0 to day 7, and the degree of cell - mediated reorganization was estimated with three separate lattice diameter measurements. RESULTS: No differences inthe initial rate of lattice reorganizatin were obeerved between fibroblasts in serum - free median with and without 20 (or 40) ng/ml rHGF groups (P ＞ 0.05);culture in the presence of serum significantly stimulated lattice reorganization( P＜0.01 ).CONCLUSION: rHGF does not modulate fibroblast- mediated extracellular matrix reorganization. This study does not provide the evidence for the autocrine of HGF/SF by gingival fibroblasts.
Keywords：hepatocyte growth factor/scatter factorftbroblast populated collagen latticegingival fibroblastcell culture
Publication Date：2000-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 262-264 )