A study of intracellular calcium ion release and its channel stimulated by calcium hydroxide in human pulp cells in vitro
Abstract：AIM: To investigate intracellular calcium ion release by Ca(OH)2 stimulus in human pulp cells in vitro and to detect a system of calcium channel. METHODS: The sixth generation of human pulp cells was cultured in RPMI1640 medium with Ca(OH)2, Ca(OH)2 and heparin, Ca(OH)2 and procaine. With the Fluo- 3/AM Probe loaded, the Ca(OH)2 was added to the medium and then the intracellular calcium level was detected by laser confocal microscope. RESULTS: Intracellular calcium concentration increased in pulp cells treated with Ca(OH)2, Ca(OH)2 and procaine, while it didn' t change in with Ca(OH)2 heparin treatment. CONCLUSION: Ca(OH)2 can stimulate the intracellular calcium release channel of inositol triphosphate receptor open in pulp cells in vitro and increase the level of intracellular calcium concentration.
Keywords：pulp cellscalcium hydroxideintracellular calciumioninositol triphosphatee receptorryanodine receptor
Publication Date：2000-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 258-261 )