Correlation between miR-34a and PSPC1 expression in cancerous tissue and clinicopathological features of OSCC and its clinical value
Yan Meng
Lyu Yongli
Liu Jin
Shi Jiapei
Yi Xiaoran
Wang Lili
Wang Qiulin
Abstract：Objective To investigate the correlation and clinical value of miR-34a and PSPC1 expression in cancer-ous tissues with clinicopathological features of oral squamous cell carcinoma(OSCC).Methods A total of 101 OSCC pa-tients(OSCC group)and 68 patients with benign oral diseases(control group)treated at Union Hospital,Tongji Medical Col-lege,Huazhong University of Science and Technology from January 2020 to January 2023 were selected as the study subjects.The expression of miR-34a and PSPC1 was detected.Spearman rank correlation or Pearson correlation analysis was used to analyze their correlation with clinicopathological features.Receiver operating characteristic(ROC)curves and DeLong's meth-od were used to analyze sensitivity,specificity,and AUC.Cox regression analysis was used to identify adverse prognostic risk factors.Kaplan-Meier curves were used for survival analysis.Results MiR-34a expression in OSCC tissue was lower than that in adjacent normal tissue and the control group,while PSPC1 expression was higher than that in adjacent normal tissue and the control group(F/P=401.394/＜0.001,215.425/＜0.001).Patients with tTMB ≥20 mut/Mb,ctDNA positive,CTC posi-tive,moderately or poorly differentiated tumors,and TNM stage Ⅲ-Ⅳ showed lower miR-34a expression than patients with tTMB＜20 mut/Mb,ctDNA negative,CTC negative,well-differentiated tumors,and TNM stage Ⅰ-Ⅱ(t/P=2.919/0.004,3.881/＜0.001,5.937/＜0.001,4.223/＜0.001,8.371/＜0.001).PSPC1 expression was higher in patients with tTMB ≥20 mut/Mb,ctDNA positive,CTC positive,moderately or poorly differentiated tumors,and TNM stage Ⅲ-Ⅳ(t/P=4.226/＜0.001,5.177/＜0.001,6.923/＜0.001,5.024/＜0.001,6.127/＜0.001).In the OSCC group,miR-34a expression was negatively correlated with tTMB,ctDNA,CTCs,pathological grade,and TNM stage(rs/P=-0.659/0.021,-0.617/0.035,-0.645/0.018,-0.629/0.007,-0.627/0.029).PSPC1 expression was positively correlated with tTMB,ctDNA,CTCs,pathological grade,and TNM stage(rs/P=0.605/0.011,0.633/0.003,0.645/0.031,0.619/0.029,0.644/0.025).The combination of miR-34a and PSPC1 was significantly more effective than either marker alone in predicting poor prognosis in patients with oral squamous cell carcinoma(Z/P=7.012/＜0.05,6.848/＜0.05).MiR-34a ≤ 0.79,PSPC1 ≥0.86,tTMB ≥20 mut/Mb,ctDNA positive,CTC pos-itive,moderately to poorly differentiated pathological grade,and TNM stage Ⅲ-Ⅳ were risk factors for poor prognosis of oral squamous cell carcinoma[HR(95％CI)=4.162(1.322-7.002),3.357(1.276-5.438),2.517(1.089-3.945),2.447(1.013-3.882),2.748(1.127-4.370),1.844(1.056-2.632),2.106(1.101-3.112)].OSCC patients with miR-34a ≤0.79 and PSPC1 ≥0.86 had a significantly lower median survival than those with miR-34a＞0.79 or PSPC1＜0.86(Log Rank x2=9.421,P＜0.001).Con-clusion The expression of miR-34a and PSPC1 in cancerous tissue is significantly associated with disease severity,poor prognosis,and overall survival in OSCC.Combined detection of both markers has greater clinical value in OSCC.
Keywords：Oral squamous cell carcinomaMicro RNA-34aPSPC1Clinicopathological characteristicsClinical value
Publication Date：2026-03-18
Online Publishing Date：2026-04-08(First online date of this platform, not the publication date of the document)
Pages：6( 335-340 )