Expression of ATAD3A and OTUD1 in cervical cancer and their clinical prognostic significance
Li Zhongyi
Lu Xi-aoyuan
Sun Jieyun
Jing Li
Yang Guochen
Abstract：Objective To analyze the expression of ATPase family AAA domain containing 3A(ATAD3A)and OTU deubiquitinase 1(OTUD1)in cervical cancer tissues and their clinical prognostic value.Methods A total of 162 pa-tients with cervical cancer admitted to the Affiliated Hospital of Xuzhou Medical University from April 2020 to April 2021 were selected.The expression levels of ATAD3A mRNA and OTUD1 mRNA in cervical cancer tissues and adjacent normal tissues were analyzed using the TCGA database.ATAD3A and OTUD1 expression were detected by qPCR and immunohisto-chemistry.The chi-square test was used to compare the protein expression of ATAD3A and OTUD1 in cervical cancer patients with different clinical parameters.Kaplan-Meier curve and Cox regression analysis were used to analyze the prognosis of cer-vical cancer.Results Analysis of the TCGA database showed that the expression levels of ATAD3A mRNA(5.44±0.76 vs 4.16±0.27)and OTUD1 mRNA(5.81±0.57 vs 4.61±0.85)in cancer tissues were significantly higher than in normal tissues(t=27.762,20.511,all P＜0.001).The mRNA and protein expression levels of both ATAD3A and OTUD1 were significantly elevated in cervical cancer tissues(t/x2=24.739,152.254,all P＜0.001).The positive rates of ATAD3A and OTUD1 were high-er in cervical cancer tissues with FIGO stage ⅠB2-ⅡB and lymph node metastasis(x2=4.000,6.359,all P＜0.05).The 3-year progression-free survival rates in the ATAD3A-positive and OTUD1-positive groups were 67.39％(99/120)and 70.19％(105/126),respectively,which were significantly lower than the 89.29％(39/42)and 88.64％(33/36)in the negative groups(Log-rank x2=4.432,4.608,P=0.035,0.027).FIGO stage,lymph node metastasis,ATAD3A,and OTUD1 were identi-fied as independent prognostic factors for cervical cancer[HR(95％CI)=1.550(1.213-1.980),1.415(1.168-1.714),1.374(1.108-1.705),1.279(1.046-1.560)].Conclusion The expressions of ATAD3A and OTUD1 are increased in cervical cancer,which are associated with tumor progression and serve as prognostic factors for cervical cancer.
Keywords：Cervical cancerATPase family AAA domain containing 3AOTU deubiquitinase 1Risk factorProg-nosis
Publication Date：2025-12-18
Online Publishing Date：2026-01-08(First online date of this platform, not the publication date of the document)
Pages：6( 1490-1495 )