The relationship between serum miR-744-5p,miR-342-3p,miR-769-5p expression with clinicopathological features and prognosis in prostate cancer patients
Wang Dong
Jin Meng
Zhao Yongxian
Li Xin
Wang Min
Cai Zhixuan
Li Anan
Qiang Jialuo
Abstract：Objective To investigate the relationship between the expression of serum microRNA-744-5p(miR-744-5p),microRNA-342-3p(miR-342-3p),and microRNA-769-5p(miR-769-5p)with clinicopathological features and prognosis in prostate cancer patients.Methods A total of 96 patients diagnosed with prostate cancer by pathological examination and ad-mitted to the First Ward of Urology,Xi'an International Medical Center Hospital from March 2019 to March 2021 were se-lected as the cancer group.The prostate cancer patients were followed up for 3 years and divided into a poor prognosis sub-group(25 cases)and a good prognosis subgroup(71 cases)based on outcomes.Additionally,96 patients with benign prostatic hyperplasia during the same period were selected as the benign group,and 96 healthy volunteers as the healthy control group.QRT-PCR was used to detect the relative expression levels of miR-744-5p,miR-342-3p,and miR-769-5p.The Kaplan-Meier method was applied to analyze the relationship between serum levels of these miRNAs and prognosis.ROC curve analysis was used to evaluate the predictive value of serum miR-744-5p,miR-342-3p,and miR-769-5p for poor prognosis in prostate cancer patients.Results Compared with the control group,the levels of miR-744-5p,miR-342-3p,and miR-769-5p in the se-rum of the benign and cancer groups were sequentially decreased(F/P=126.382/＜0.001,76.464/＜0.001,87.597/＜0.001).There were no significant differences in serum levels of miR-744-5p,miR-342-3p,and miR-769-5p across lymph node metastasis,vascular invasion,clinical stages,or differentiation degrees.Compared with the good prognosis group,serum levels of miR-744-5p,miR-342-3p,and miR-769-5p in the poor prognosis group were significantly reduced(t/P=7.133/＜0.001,6.312/＜0.001,6.372/＜0.001).The 3-year survival rate in the miR-744-5p high expression group[45/48(93.75％)]was higher than that in the low expression group[26/48(54.17％)](Log-rank x2=376.516,P＜0.001);the 3-year survival rate in the miR-342-3p high expression group[46/48(95.83％)]was higher than that in the low expression group[25/48(52.08％)](Log-rank x2=24.986,P＜0.001);the 3-year survival rate in the miR-769-5p high expression group[44/47(93.62％)]was higher than that in the low expression group[27/49(55.10％)](Log-rank x2=19.295,P＜0.001).Compared with individual detection of serum miR-744-5p,miR-342-3p,and miR-769-5p,the combined detection of all three significantly increased the AUC for predicting prognosis in prostate cancer patients(Z=2.705,P=0.007;Z=3.424,P=0.001;Z=3.053,P=0.002).Con-clusion The levels of miR-744-5p,miR-342-3p,and miR-769-5p in the serum of prostate cancer patients are significantly re-duced,and their combined detection can improve the predictive value for prognosis in prostate cancer patients.
Keywords：Prostate cancerMicroRNA-744-5pMicroRNA-342-3pMicroRNA-769-5pClinicopathological fea-turesPrognosis
Publication Date：2025-12-18
Online Publishing Date：2026-01-08(First online date of this platform, not the publication date of the document)
Pages：6( 1485-1489,1495 )