LncRNA RMRP inhibits lipopolysaccharide-induced renal tubular epithelial cell injury by targeting miR-580-3p/RhoA axis
Han Lingzhi
Zhang Xue
Lu Xiangyun
Liu Kun
Shao Chunzhi
Abstract：Objective To investigate whether long non-coding RNA(LncRNA)mitochondrial RNA processing RNA endonuclease RNA component(RMRP)can inhibit lipopolysaccharide(LPS)-induced renal tubular epithelial cell injury by tar-geting the microRNA(miR)-580-3p/Ras homologous gene family member A(RhoA)axis.Methods The experiment was conducted in the Central Laboratory of Jiaozhou Central Hospital of Qingdao from May to October 2024.Normally cultured human renal tubular epithelial cells HK-2 were designated as the Control group.A cell injury model was established using LPS,and the cells were divided into the Model group,sh-NC group,sh-RMRP group,sh-RMRP+anti-NC group,and sh-RMRP+anti-miR-580-3p group according to different treatments.The expression levels of LncRNA RMRP,miR-580-3p,and RhoA mRNA were detected by quantitative real-time PCR(qRT-PCR).The regulatory relationships between LncRNA RMRP and miR-580-3p,and between miR-580-3p and RhoA were examined by dual-luciferase assay.Cell proliferation was assessed by Cell Counting Kit-8(CCK-8)assay.The levels of interleukin-1β(IL-1β),interleukin-6(IL-6),and tumor necrosis factor-α(TNF-α)were measured by enzyme-linked immunosorbent assay(ELISA).Apoptosis was analyzed by flow cytometry.The protein expressions of RhoA,proliferating cell nuclear antigen(PCNA),B-cell lymphoma-2(Bcl-2),and Bcl-2 associated X protein(Bax)were detected by Western blot.Results Compared with the Control group,the Model group showed increased expression of LncRNA RMRP,RhoA mRNA and protein,apoptosis rate,IL-1β,IL-6,TNF-α,and Bax protein expression,while miR-580-3p expression,HK-2 cell survival rate,and PCNA and Bcl-2 protein expression decreased(P＜0.01).Compared with the Model group and sh-NC group,the sh-RMRP group exhibited decreased expression of LncRNA RMRP,RhoA mRNA and protein,apoptosis rate,IL-1β,IL-6,TNF-α,and Bax protein expression,while miR-580-3p expression,HK-2 cell survival rate,and PCNA and Bcl-2 protein expression increased(P＜0.01).Compared with the sh-RMRP group and sh-RMRP+anti-NC group,the sh-RMRP+anti-miR-580-3p group showed increased expression of LncRNA RMRP,RhoA mRNA and protein,apoptosis rate,IL-1β,IL-6,TNF-α,and Bax protein expression,while miR-580-3p expression,HK-2 cell survival rate,and PCNA and Bcl-2 protein expression decreased(P＜0.01).The dual-luciferase assay showed that,compared with the mimic-NC group,the luciferase activity in the miR-580-3p mimic group was decreased(P＜0.01).Conclusion Knockdown of LncRNA RMRP inhibits the LPS-induced inflammatory response and apoptosis in HK-2 cells by targeting the miR-580-3p/RhoA axis,thereby exerting a protective effect against renal tubular epithelial cell injury.
Keywords：Acute renal injuryRenal tubular epithelial cellLncRNA RMRPmiR-580-3p/RhoA axisLipopolysac-charide
Publication Date：2025-11-18
Online Publishing Date：2025-12-05(First online date of this platform, not the publication date of the document)
Pages：9( 1369-1377 )