The correlation between peripheral blood Omentin-1,EphB2 and major adverse limb events in patients with lower limb peripheral arterial disease after interventional therapy

Liu Bo; Li Wei; Man Wenling; Dong Chang; Yao Gong-zan; Yang Po

doi:10.3969/j.issn.1671-6450.2025.10.015

Abstract：Objective To investigate the correlation between peripheral blood omentin-1(Omentin-1),ephrinB2(EphB2)levels and major adverse limb events in patients with peripheral arterial disease(PAD)after interventional therapy.Methods From June 2021 to June 2023,150 patients with PAD of lower extremity who were admitted to the Interventional Department of Harbin Medical University Affiliated Second Hospital for Interventional Therapy were selected as the case group,and 60 healthy subjects in the hospital during the same period were selected as the healthy control group.The case group was divided into MALE subgroup(n=35)and non-MALE subgroup(n=1 15)according to whether major adverse limb events(MALE)occurred within 12 months.The levels of Omentin-1 and EphB2 in peripheral blood were compared between the case group and the healthy control group.Pearson correlation analysis was used to analyze the correlation between the lev-els of Omentin-1 and EphB2 in peripheral blood and CRP,TNF-α and ABI in PAD patients;Multivariate Logistic regression analysis was used to analyze the influencing factors of MALE in PAD patients after interventional therapy;The receiver oper-ating characteristic curve(ROC)was used to analyze the predictive value of peripheral blood omentin-1 and EphB2 levels for MALE.Results The serum Omentin-1 level in the case group was lower than that in the healthy control group,and the plas-ma EphB2 level was higher than that in the healthy control group(t=-16.083,24.454,all P＜0.001);The level of Omentin-1 was negatively correlated with CRP and TNF-α,but positively correlated with ABI value;EphB2 level was positively correlated with CRP and TNF-α,but negatively correlated with ABI(r=-0.564,-0.493,0.602,0.397,0.560,-0.558,all P＜0.05).High ABI and high Omentin-1 were independent protective factors for MALE after PAD interventional therapy,while high EphB2 was an independent risk factor[OR(95％CI)=0.802(0.655-0.981),0.613(0.444-0.845),2.335(1.200-4.561)].The AUC of Omentin-1,EphB2 and their combination in predicting the occurrence of MALE after PAD interventional therapy were 0.789,0.715 and 0.889,respectively.The combination of the two was superior to their respective predictive values(Z=2.065,3.177,P=0.039,0.001).Conclusion The levels of Omentin-1 and EphB2 in peripheral blood are abnormally expressed in PAD patients,which are related to the inflammatory response and severity of PAD patients.The combination of the two is helpful to improve the predictive value of MALE after PAD interventional therapy.