Effect of PRC1 overexpression on proliferation and apoptosis in chemotherapy-damaged ovarian granulosa cells
Xu Shuli
Huang Xinyi
Zeng Wenzhuo
Sun Xiaofeng
Abstract：Objective To study the effects of protein regulator of cytokinesis 1(PRC1)overexpression on prolifera-tion and apoptosis in ovarian granulosa cells(GCs)damaged by phosphoramide mustard(PM).Methods From April 2023 to January 2024,conducted experiments in the laboratory of the Medical College of Hunan University of Chinese Medicine.Pri-mary rat GCs were treated with PM to simulate chemotherapy injury.Lentiviral transfection achieved PRC1 overexpression.Groups:OE-NC(empty vector),OE-NC+PM,OE-PRC1,OE-PRC1+PM.CCK-8,EdU staining,flow cytometry,and Western blot assessed proliferation,apoptosis,and protein levels.Results PM reduced cell viability,EdU+cells,Bcl-2,and PRC1 while increasing Bax and Cleaved-Caspase-3(all P＜0.01).PRC1 overexpression reversed these effects(P＜0.01 or P＜0.05)and accelerated S-phase progression.OE-PRC1+PM showed lower apoptosis than OE-NC+PM(P＜0.01).Conclusion PRC1 overexpression promotes proliferation and inhibits apoptosis in chemotherapy-damaged GCs.
Keywords：Ovarian granulosa cellsChemotherapy damageProtein regulator of cytokinesis 1Phosphoramide mus-tardProliferationApoptosis
Publication Date：2025-09-18
Online Publishing Date：2025-10-11(First online date of this platform, not the publication date of the document)
Pages：6( 1122-1127 )