Expression of P4HA1 and HMGCS1 in nasopharyngeal carcinoma and their correlation with stemness-related genes and prognostic significance
Zhang Lijie
Peng Jun
Liu Shuhong
Dai Chenglin
Abstract：Objective To investigate the expression of prolyl 4-hydroxylase subunit α1(P4HA1)and 3-hydroxy-3-methylglutaryl-CoA synthase 1(HMGCS1)in nasopharyngeal carcinoma(NPC)and their relationship with stemness-related genes and prognosis.Methods From June 2016 to January 2020,122 NPC tissues and adjacent tissues were collected.RT-qPCR measured mRNA levels of P4HA1,HMGCS1,and stemness-related genes(OCT4,SOX2,NANOG).Immunohisto-chemistry detected protein expression.Pearson correlation analyzed relationships between P4HA1/HMGCS1 and stemness genes.Kaplan-Meier and Cox regression evaluated prognostic factors.Results NPC tissues showed higher P4HA1,HMGCS1,OCT4,SOX2,and NANOG mRNA levels than adjacent tissues(t/P=44.831/＜0.001,45.243/＜0.001,44.210/＜0.001,50.142/＜0.001,40.642/＜0.001).P4HA1 and HMGCS1 mRNA positively correlated with stemness genes(r/P=0.712,0.685,0.739,0.651,0.711,0.764,all P＜0.001).Protein positivity rates for P4HA1(68.85％vs.8.20％)and HMGCS1(67.21％vs.7.38％)were higher in NPC(x2/P=94.762/＜0.001,93.391/＜0.001).TNM Ⅲ-Ⅳ patients had higher P4HA1/HMGCS1 positivity(x2/P=30.417/＜0.001,12.594/＜0.001).P4HA1/HMGCS1 positivity predicted poorer 5-year survival(60.71％vs.86.84％;59.76％vs.87.50％;Log-rank x2=8.154/0.004,9.018/0.003).TNM Ⅲ-Ⅳ,P4HA1+,and HMGCS1+were independent risk factors[HR=1.759(1.227-2.523),1.861(1.313-2.638),1.315(1.151-1.503)].Conclusion P4HA1 and HMGCS1 are upregulated in NPC,associated with stemness genes and TNM stage,serving as novel prognostic markers.
Keywords：Nasopharyngeal carcinomaProlyl 4-hydroxylase subunit α13-hydroxy-3-methylglutaryl-CoA synthase 1Stemness-related genesPrognosis
Publication Date：2025-09-18
Online Publishing Date：2025-10-11(First online date of this platform, not the publication date of the document)
Pages：6( 1109-1114 )