Association of serum miR-106a-5p and PFKFB3 levels with disease severity and prognosis in sepsis patients
Jiang Wenjuan
Ren Yang
Li Baiyuan
Li Tingting
Zheng Yanni
Abstract：Objective To investigate miR-106a-5p and 6-phosphofructo-2-kinase(PFKFB3)as sepsis biomarkers.Methods A total of 152 sepsis patients admitted to the ICU of the Affiliated Hospital of Yan'an University between January 2022 and September 2024 were selected as the sepsis group.Based on disease severity,they were divided into a general sepsis subgroup(n=84)and a septic shock subgroup(n=68).According to 90-day outcomes,patients were further categorized into a survival group and a death group.Additionally,76 healthy volunteers undergoing routine physical examinations during the same period were included as the healthy control group.Serum levels of miR-106a-5p and PFKFB3 were measured using real-time quantitative PCR and ELISA,respectively.The binding site between miR-106a-5p and the 3'-UTR of PFKFB3 was pre-dicted using an online database.Pearson correlation analysis was used to assess the relationship between serum miR-106a-5p and PFKFB3 levels.Multivariate logistic regression was performed to identify independent prognostic factors,and ROC curves were used to evaluate the predictive value of serum miR-106a-5p and PFKFB3 levels.Results Compared with the healthy control group,the sepsis group showed significantly lower serum miR-106a-5p levels and higher PFKFB3 levels(t/P=22.855/＜0.001,17.897/＜0.001).A binding site was identified at position 1506-1512 of the PFKFB3 3'-UTR.Serum miR-106a-5p levels were negatively correlated with PFKFB3 levels in sepsis patients(r/P=0.669/＜0.001).Compared with the gen-eral sepsis subgroup,the septic shock subgroup had lower miR-106a-5p levels and higher PFKFB3 levels(t/P=7.025/＜0.001,6.770/＜0.001).The 90-day mortality rate was 39.47％(60/152).Septic shock,higher SOFA scores,higher APACHE Ⅱscores,and elevated PFKFB3 were identified as independent risk factors for death,whereas high miR-106a-5p was an inde-pendent protective factor[OR(95％CI)=7.933(1.932-32.570),1.262(1.104-1.443),1.070(1.025-1.116),1.034(1.013-1.055),0.850(0.785-0.920)].The AUCs for predicting prognosis using serum miR-106a-5p,PFKFB3,and their combination were 0.790,0.782,and 0.892,respectively,with the combined prediction outperforming either marker alone(Z/P=3.388/0.001,3.695/＜0.001).Conclusion The miR-106a-5p/PFKFB3 axis correlates with sepsis severity and outcomes,offering synergistic prognostic value.
Keywords：SepsismiR-106a-5p6-phosphofructo-2-kinase/fructose-26-biphosphatase 3Prognosis
Publication Date：2025-09-18
Online Publishing Date：2025-10-11(First online date of this platform, not the publication date of the document)
Pages：6( 1086-1091 )