Mechanistic study of Jinlida Granules in the treatment of type 2 diabetes:A network pharmacology and molecular docking approach

Cheng Si; Zhang Yanping; Liu Xin; Wang Fei; Zhao Jindong; Fang Zhaohui

doi:10.3969/j.issn.1671-6450.2025.09.011

Abstract：Objective This study aims to comprehensively explore the active components,key targets,and signaling pathways involved in the mechanism of Jinlida Granules in treating type 2 diabetes mellitus(T2DM)using network pharma-cology and molecular docking techniques.Methods Active ingredients and their corresponding targets of Jinlida Granules were retrieved from the TCMSP database,while T2DM-related targets were obtained from DrugBank and GeneCards databas-es.The intersecting targets between the drug and the disease were identified and used to construct a protein-protein interaction(PPI)network to identify critical targets.The top five targets based on degree values were selected as core targets for T2DM treatment.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses were performed using the DAVID database.Finally,molecular docking was employed to validate the binding affinity between the top active ingredients and their respective targets.Results A total of 171 active ingredients in Jinlida Granules were identified.Quercetin,luteolin,stigmasterol,kaempferol,and β-sitosterol were identified as the five key compounds with significant potential in managing T2DM.Through target screening,922 T2DM-related targets were identified,with 153 intersecting targets.TNF,IL-6,Akt1,PPARG,and TP53 were identified as the key targets.GO analysis revealed that the associated biological processes were mainly related to inflammation,hypoxic response,cell growth,and apoptosis.KEGG pathway enrichment analysis high-lighted the TNF signaling pathway,among others,as crucial in the therapeutic effects of Jinlida Granules on T2DM.Molecular docking demonstrated strong and stable interactions between the key active ingredients and their respective targets.Conclusion Jinlida Granules exert therapeutic effects on T2DM through the synergistic modulation of multiple targets and signaling path-ways.This study provides a theoretical basis for the use of Jinlida Granules in T2DM treatment,offers new insights for future diabetes research,and contributes to the integration of traditional Chinese medicine with modern scientific approaches.