The relationship between serum MIC-1,CGRP and Helicobacter pylori infection in patients with chronic gastritis and their clinical significance

Su Quanqiu; Shi Niu; Yang Ting; Yu Jing; Zhang Miao

doi:10.3969/j.issn.1671-6450.2025.08.011

Abstract：Objective To investigate the relationship and clinical significance between serum macrophage inhibitory factor-1(MIC-1),calcitonin gene-related peptide(CGRP)and Helicobacter pylori(Hp)infection in patients with chronic gas-tritis.Methods From June 2021 to June 2024,104 patients diagnosed with chronic gastritis in our hospital were included as the chronic gastritis group.Based on the results of the 13 C urea breath test(13 C-UBT)breath test,they were assigned into the Hp subgroup(64 cases)and the non Hp subgroup(40 cases).Ninety healthy individuals were simultaneously selected as the healthy group.13 C-UBT retesting was used to confirm the prognosis of eradication treatment in Hp subgroup patients.Enzyme linked immunosorbent assay(ELISA)was applied to detect serum levels of MIC-1,CGRP,and inflammatory factors interleukin-2(IL-2)and interleukin-6(IL-6).Pearson and Spearman correlation were applied to analyze the correlation between serum MIC-1,CGRP levels with IL-6,Hp infection,and prognosis.Multivariate Logistic regression was applied to analyze the influencing factors of Hp infection in patients with chronic gastritis.ROC was applied to analyze the predictive value of serum MIC-1 and CGRP for Hp infection in patients with chronic gastritis.Results The serum MIC-1 level in chro-nic gastritis group was higher than that in healthy control group,and the serum CGRP level was lower than that in healthy control group(t/P=15.529/＜0.001,33.596/＜0.001).The serum IL-6 and MIC-1 levels of Hp subgroup were higher than those of non-Hp subgroups,and the serum IL-2 and CGRP levels were lower than those of non-Hp subgroups(t/P=23.282/＜0.001,5.501/＜0.001,5.968/＜0.001,6.925/＜0.001).After Hp eradication treatment,the serum MIC-1 level in the non-eradication group was higher than that in the eradication group,and the serum CGRP level was lower(t/P=2.667/0.008,6.646/＜0.001).The serum MIC-1 level was positively correlated with IL-6 and Hp infection,and negatively correlated with prognosis(r/P=0.544/＜0.001,0.764/＜0.001,-0.487/＜0.001).The level of serum CGRP was negatively correlated with IL-6 levels and Hp in-fection,and positively correlated with prognosis(r/P=-0.572/＜0.001,-0.654/＜0.001,0.682/＜0.001).And positively correlated with prognosis.MIC-1,IL-6,and CGRP were influencing factors of Hp infection in patients with chronic gastritis[OR(95％CI)=0.835(0.750-0.901),0.840(0.755-0.904),0.913(0.841-0.959)].Serum MIC-1 and CGRP could be used separately to diag-nose Hp infection in patients with chronic gastritis,and the combined diagnostic effect of the two was greater than the individ-ual predictive value(Z/P=2.342/0.019,2.197/0.028).Conclusion Serum MIC-1,CGRP,and inflammatory factor IL-6 are all factors that affect the infection of Hp in patients with chronic gastritis.Among them,serum MIC-1 and CGRP can diagnose whether patients with chronic gastritis are infected with Hp,and the combination of the two has a better diagnostic effect.