The relationship between the expression of NSUN6 and SULF1 in cervical cancer tissues and clinicopathological fea-tures and prognostic value
Zhang Yi
Wu Zirui
Li Yujia
Huang Guanyou
Abstract：Objective To study the expression ofNOP2/Sun RNA methyltransferase 6(NSUN6)and Sulfatase 1(SULF1)in cervical cancer tissues and their relationship with clinicopathological features and prognosis.Methods The ex-pression differences of NSUN6 and SULF1 in cervical cancer tissues and normal cervical tissues in the human genome atlas database were analyzed by R language.The clinical data of 92 patients with cervical cancer admitted to the Department of gy-necology and obstetrics of Guizhou Medical University Affiliated Hospital from June 2019 to June 2021 were collected.The expression of NSUN6 and SULF1 in cancer tissues was detected by real-time fluorescence quantitative PCR and immunohisto-chemistry;K-M curve was used to compare the prognostic differences of different NSUN6 and SULF1 protein expressions in patients with cervical cancer;Cox regression model was used to screen the prognostic factors of cervical cancer.Results The results of TCGA database and qPCR showed that the expression of NSUN6 mRNA and SULF1 mRNA in cervical cancer tis-sues was higher than that in normal tissues(t/P=22.281/＜0.001,53.721/＜0.001,37.619/＜0.001,36.442/＜0.001);The positive rates of NSUN6 and SULF1 proteins in cervical cancer tissues were higher than those in adjacent tissues(t/P=85.546/＜0.001,86.891/＜0.001).The positive rates of NSUN6 and SULF1 in cervical cancer tissues with FIGO stage ⅠB2-ⅡB and lymph node metastasis were higher than those of ⅠA-ⅠB1 and no lymph node metastasis(t/P=7.934/0.005,5.327/0.021,13.512/＜0.001,4.564/0.033).There was no significant difference in 3-year overall survival rate between NSUN6 positive and negative,SULF1 positive and negative(P＞0.05);The 3-year progression-free survival rate of NSUN6 positive and SULF1 positive was lower than that of NSUN6 negative and SULF1 negative(x2/P=8.120/0.004,36.442/＜0.001).NSUN6 positive,SULF1 posi-tive,FIGO stage ⅠB2-ⅡB and lymph node metastasis were risk factors affecting the prognosis of cervical cancer[HR(95％CI)=1.374(1.082-1.746),1.278(1.054-1.548),1.402(1.115-1.764),1.339(1.094-1.639)].Conclusion The expressions of NSUN6 and SULF1 mRNA and protein in cervical cancer tissues are increased,both of which are related to poor clinicopatho-logical features and are markers for evaluating the prognosis of cervical cancer.
Keywords：Cervical cancerNOP2/SUN RNA methyltransferase family member 6Sulfatase 1Prognosis
Publication Date：2025-07-18
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 843-847,854 )