The relationship between Sema3A,CCAT2 levels in primary laryngeal carcinoma and clinicopathological features and prognosis
Tian Ye
Li Bei
Pan Qingchun
Zhao Haiqi
Abstract：Objective To study the expression of serum semaphorin3a(Sema3A),long non coding RNA colon canc-er associated transcript 2(CCAT2)and their clinical significance in patients with primary laryngeal cancer(PLC),and to ana-lyze the prognostic value.Methods From February 2019 to February 2021,104 patients with PLC who underwent surgical treatment(partial or total laryngectomy)were admitted to the department of otolaryngology and head and neck surgery at the Affiliated Hospital of North Sichuan Medical College.The expression of Sema3 A mRNA,CCAT2 in tissues was detected by qPCR.Kaplan Meier curve and Cox regression were used to analyze the prognostic factors.Results The levels of Sema3A mRNA and CCAT2 in cancer tissues were higher than those in adjacent tissues,and the difference was statistically significant(t=38.470,35.015,P＜0.001).The expression of Sema3A mRNA in PLC cancer tissue was significantly positively correlated with CCAT2(r=0.678,P＜0.001).The levels of Sema3A mRNA and CCAT2 in PLC patients with TNM stage Ⅲ-Ⅳ,lymph node metastasis were higher(t=8.986,10.526,11.195,5.225,P＜0.001).The area under the curve(95％CI)of the combination of Sema3A mRNA and CCAT2 for the prognosis of PLC patients was 0.905(0.880-0.948),which was higher than that of Sema3A mRNA[0.844(0.809-0.887)]and CCAT2[0.815(0.781-0.852)],the difference was statistically sig-nificant(Z=4.641,4.910,P＜0.001).The 3-year overall survival rate in the high expression group of Sema3A was 63.42％(26/41),which was lower than 95.24％(60/63)in the low expression group(log rank x2=10.680,P=0.001);The 3-year over-all survival rate in the high expression group of CCAT2 was 67.50％(27/40),which was lower than 92.19％(59/64)in the low expression group(log rank x2=11.870,P＜0.001).TNM stage,lymph node metastasis,Sema3A mRNA and CCAT2 were the factors affecting the prognosis of PLC[OR(95％CI)=1.458(1.106-1.922),1.433(1.128-1.821),1.377(1.125-1.685),1.315(1.105-1.565)].Conclusion Elevated levels of Sema3A mRNA and CCAT2 in PLC are associated with adverse clini-cal pathological features and poor prognosis of PLC patients.
Keywords：Primary laryngeal carcinomaBrain signal protein 3ALong non coding RNA colon cancer related tran-script 2Prognosis
Publication Date：2025-06-18
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 728-733 )