The correlation between CPT1A,PDPK1 expression and epithelial-mesenchymal transition in prostate cancer and its clinical significance

Gu Hao; Li Bo; Su Ruiping; Guo Boxin; Wang Bao

doi:10.3969/j.issn.1671-6450.2025.06.016

Abstract：Objective To study the expression of carnitine palmitoyltransferase 1A(CPT1A)and 3-phosphoinositide dependent protein kinase 1(PDPK1)in prostate cancer and its correlation with epithelial mesenchymal transition(EMT)and its clinical significance.Method:A total of 110 patients with prostate cancer treated in the Department of Urology of Xi'an People's Hospital(Xi'an Fourth Hospital/Northwest University Affiliated People's Hospital)from January 2019 to Decem-ber 2021 were selected.The expression levels of CPT1 A mRNA,PDPK1 mRNA and EMT genes[Twist,E-cadherin(E-cad),N-cadherin(N-cad)]mRNA in tissues were detected by real-time fluorescence quantitative PCR.Pearson correlation was used to study the correlation between CPT1 A mRNA,PDPK1 mRNA and EMT gene;the expression of CPT1 A and PDPK1 protein was detected by immunohistochemistry;the effect of CPT1A and PDPK1 protein expression on the prognosis of pros-tate cancer was statistically analyzed.Results Compared with the adjacent tissues,the expression of CPT1A mRNA,PDPK1 mRNA,Twist mRNA,N-cad mRNA in cancer tissues was higher,and the expression of E-cad mRNA was lower(t/P=37.485/＜0.001,42.555/＜0.001,41.666/＜0.001,33.161/＜0.001,37.382/＜0.001);the expression of CPT1A mRNA and PDPK1 mRNA in prostate cancer was positively correlated with the expression of EMT gene Twist mRNA and N-cad mRNA(r=0.722,0.630;0.651,0.643,all P＜0.001),and negatively correlated with E-cad mRNA(r=-0.712,-0.730,all P＜0.001);the positive rates of CPT1A and PDPK1 protein in prostate cancer tissues were 70.91％(78/110)and 65.45％(72/110),which were higher than those in adjacent tissues 5.45％(6/110)and 6.36％(7/110)(x2=99.832,52.011,all P＜0.001);the positive rates of CPT1 A and PDPK1 protein in prostate cancer tissues with T3 a stage and Gleason score＞7 were increased(x2/P=9.870/0.002,7.405/0.007;13.062/＜0.001,12.351/＜0.001).The 3-year progression-free survival rates of CPT1A positive group and PDPK1 positive group were 51.28％(40/78)and 48.61％(35/72),respectively,which were lower than 81.25％(26/32)and 81.58％(31/38)of CPT1A negative group and PDPK1 negative group(Log-rank x2=7.845,10.980,P=0.005,0.001).CPT1 A positive,PD-PK1 positive,T3 a stage and Gleason score＞7 were risk factors affecting the prognosis of prostate cancer[OR(95％CI)=1.326(1.077-1.632),1.301(1.110-1.525),1.324(1.074-1.633),1.370(1.092-1.720)].Conclusion The expression of CPT1 A and PDPK1 in prostate cancer is increased,which is positively correlated with the expression of EMT gene,and is a tumor marker for evaluating the prognosis of prostate cancer.