Analysis of NLRC5 and NSUN2 expression and clinical prognostic value in endometrial cancer
Guo Yang
Zhang Fengge
Zhu Dan
Ni Qin
Abstract：Objective To investigate the relationship between nucleotide binding oligomerization domain like receptor 5(NLRC5),NOP2/Sun RNA methyltransferase family member 2(NSUN2)in endometrial cancer(EC)and clinical pathological features and prognosis.Methods One hundred and thirty-six EC patients admitted to the Department of Gyne-cology,Shunyi Maternal and Child Health Hospital of Beijing from February 2016 to February 2019 were selected.Immuno-histochemistry and qPCR were used to detect the protein and mRNA expression of NLRC5 and NSUN2 in EC cancer tissues and adjacent tissues.The differential expression of NLRC5 and NSUN2 mRNA in EC patients with different clinical patholog-ical parameters were compared.The value of NLRC5 and NSUN2 mRNA expression in evaluating the prognosis of EC pa-tients analyzed by the receiver operating characteristic curve.Kaplan Meier curves and COX regression analysis were used to investigate the factors affecting the prognosis of EC patients.Results Compared with adjacent tissues,the positivity rates of NLRC5 mRNA and protein were lower in cancer tissues,while the positivity rates of NSUN2 mRNA and protein were higher(t/x2=14.428,159.835,31.642,143.753,P＜0.001).There were significant differences in NLRC5 and NSUN2 mRNA expres-sion among EC patients with different FIGO stages and lymph node metastasis.Patients with FIGO stage Ⅲ and lymph node metastasis EC had lower NLRC5 mRNA expression and higher NSUN2 mRNA expression in cancer tissues(both P＜0.05).The optimal cutoff values for prognostic evaluation of NLRC5 and NSUN2 mRNA expression in EC cancer tissues are 2.52 and 3.83,respectively.The 5-year overall survival(OS)of the NLRC5 mRNA high and low group were 91.67％(22/24)and 73.21％(82/112),respectively,with statistically significant differences in the curves(Log Rank x2=3.402,P=0.012).The 5-year OS of the NSUN2 mRNA high and low group were 42.31％(11/26)and 84.55％(93/110),respectively,with statistically significant differences(Log Rank x2=18.910,P＜0.001).FIGO stage Ⅲ,lymph node metastasis,high NSUN2 mRNA were risk factors affecting the prognosis of EC patients,while high NLRC5 mRNA was a protective factor[HR(95％CI)=1.449(1.148-1.830),1.442(1.124-1.850),1.570(1.145-2.152),0.611(0.462-0.807)].Conclusion The decreased expression of NLRC5 and increased expression of NSUN2 in EC are associated with FIGO staging and lymph node metastasis,and are bio-markers for evaluating the prognosis of EC.
Keywords：Endometrial cancerNucleotide binding oligomerization domain like receptor 5NOP2/Sun RNA meth-yltransferase family member 2Prognosis
Publication Date：2025-06-18
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 693-698 )