The predictive value of serum circRNA ROBO2,circRNA SLC8A1,and circRNA CDR1as for major adverse cardio-vascular events after PCI in patients with acute myocardial infarction
Shao Dan
Li Yan
Zhang Ting
Xiang Haiyan
Feng Chong
Abstract：Objective To analyze the predictive value of serum circular RNA(circRNA)roundabout homolog 2(ROBO2),circRNA solute carrier family 8 member A1(SLC8A1),and circRNA cerebellar degeneration related protein 1 anti-sense(CDR1as)for major adverse cardiovascular events(MACE)in patients with acute myocardial infarction(AMI)after per-cutaneous coronary intervention(PCI).Methods Between January 2020 and December 2020,140 AMI patients who under-went PCI surgery in our hospital were included as AMI group,and 140 healthy individuals who underwent physical examina-tion were included as control group.AMI patients were assigned into MACE group and non-MACE group based on whether MACE occurred after PCI.Real-time fluorescence quantitative PCR(qRT-PCR)was used to detect serum circRNA ROBO2,circRNA SLC8A1,and circRNA CDR1as.Logistic regression was used to analyze the influencing factors of postoperative MACE.ROC curve was used to evaluate the predictive value of serum circRNA ROBO2,circRNA SLC8A1,and circRNA CDR1as for postoperative MACE in AMI patients.Results The AMI group had higher serum circRNA ROBO2,circRNA SLC8A1,and circRNA CDR1as than control group(t/P=9.192/＜0.001,10.903/＜0.001,11.152/＜0.001).The MACE group had higher serum circRNA ROBO2,circRNA SLC8A1,and circRNA CDR1as than non-MACE group(t/P=5.655/＜0.001,7.935/＜0.001,5.797/＜0.001).There was a statistically conspicuous difference in the number of coronary artery lesions and no reflow during surgery between MACE group and non-MACE group(X2/P=14.799/＜0.001,8.057/＜0.001).Logistic regression analysis revealed that the number of coronary artery lesions ≥2,no reflow during surgery,serum circRNA ROBO2,circRNA SLC8A1,and circRNA CDR1as were the influencing factors for MACE in AMI patients after PCI[OR(95％CI)=4.639(1.970-10.925),4.722(1.917-11.633),5.337(1.949-14.616),5.082(1.798-14.361),4.734(1.925-11.640)].The AUC pre-dicted by the joint of the three was conspicuously greater than that predicted by circRNA ROBO2,circRNA SLC8A1,and cir-cRNA CDR1as alone(Z=2.660,2.321,2.740,P=0.008,0.020,0.006).Conclusion Serum circRNA ROBO2,circRNA SLC8A1,and circRNA CDR1as are conspicuously elevated in AMI patients,and the joint of the three has good predictive val-ue for MACE after PCI in patients.
Keywords：Acute myocardial infarctionCircular RNA roundabout homolog 2CircRNA solute carrier family 8 member A1CircRNA cerebellar degeneration related protein 1 antisensePercutaneous coronary interventionMajor adverse cardiovascular events
Publication Date：2025-06-18
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 661-666 )