Mechanism of Yixinyin alleviating myocardial ischemia-reperfusion injury based on network pharmacology
Yue Wen-heng
Huang Kun
Wu Yue
Wen Jiayu
Liang Chun
Abstract：Objective To investigate the roe of Yixinyin in alleviating myocardial ischemia-reperfusion injury(MI-RI)and elucidate its potential molecular mechanisms using a network pharmacology approach.Methods The active compo-nents of Yixinyin and their target genes were identified through the TCMSP and BATMAN databases.MIRI-related target genes were collected from the GeneCards,OMIM,and HPO databases.Intersection analysis was performed to construct a"Yixinyin-active component-MIRI common target"network.Subsequently,a protein-protein interaction(PPI)network was de-veloped using the STRING database and visualized with Cytoscape to identify the core target genes,followed by GO and KEGG enrichment analyses.Molecular docking was conducted between the core target genes and the key active components,with cellular model validation performed.Results A total of 146 common target genes for Yixinyin and MIRI were identi-fied,with 18 core target genes further confirmed.GO and KEGG enrichment analyses revealed that these targets are mainly involved in oxidative stress and inflammation-related pathways.Molecular docking indicated the strong binding affinity be-tween the active components of Yixinyin and the core target genes.Cell experiments have shown that Yixinyin inhibits car-diomyocyte apoptosis under ischemic and hypoxic conditions.Conclusion Yixinyin may alleviate MIRI through a synergis-tic effect on multiple targets and pathways.
Keywords：YixinyinMyocardial ischemia-reperfusion injuryProtein-protein interaction networkMolecular doc-king
Publication Date：2025-05-18
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 601-607 )