Alterations and clinical significance of IL-8 and CXCR2 levels in peripheral blood CD34+cells of patients with myelo-dysplastic syndrome

Huang Qin; Zhai Shunsheng; Lin Siying; An Li

doi:10.3969/j.issn.1671-6450.2025.05.013

Abstract：Objective To investigate the alterations and clinical significance of interleukin-8(IL-8)and CXC che-mokine receptor 2(CXCR2)levels in peripheral blood CD34+cells of patients with myelodysplastic syndrome(MDS).Methods Clinical data from 100 MDS patients admitted to the Department of Hematology,People's Hospital of Xinjiang Uygur Autonomous Region,from January 2021 to February 2024 were retrospectively analyzed.Patients were grouped ac-cording to the Revised International Prognostic Scoring System(IPSS-R):the low-risk MDS group(n=50)included very low-,low-,and intermediate-risk patients,while the high-risk MDS group(n=50)included high-and very high-risk patients,and 20 healthy subjects were selected as healthy control group.Reverse transcription-polymerase chain reaction(RT-PCR)was used to measure IL-8 and CXCR2 mRNA expression in peripheral blood CD34+cells,while Western blot(WB)was employed to determine IL-8 and CXCR2 protein levels.Differences in IL-8 and CXCR2 expression among groups were compared.Pearson correlation analysis was performed to assess the relationship between IL-8 and CXCR2 in MDS patients.Receiver operating characteristic(ROC)curve analysis was conducted to evaluate the diagnostic value of IL-8 and CXCR2 mRNA in peripheral blood CD34+cells for MDS.Results IL-8 and CXCR2 mRNA expression levels followed the order:high-risk MDS group＞low-risk MDS group＞healthy control group(F/P=351.477/＜0.001,872.932/＜0.001).IL-8 and CXCR2 protein levels in peripheral blood CD34+cells followed the same trend:high-risk MDS group＞low-risk MDS group＞healthy control group(F/P=278.583/＜0.001,455.498/＜0.001).Pearson correlation analysis revealed a positive correlation between IL-8 and CXCR2 in peripheral blood CD34+cells of MDS patients(r/P=0.419/0.023).The area under the curve(AUC)for diagnosing MDS using IL-8 mRNA,CXCR2 mRNA,and their combination was 0.754,0.801,and 0.842,respectively.The combined detection outperformed individual assessments(Z/P=2.345/0.019,2.750/0.006).Conclusion The expression levels of IL-8 and CXCR2 in peripheral blood CD34+cells of MDS patients were significantly higher than those in healthy controls and correlated with prognostic risk stratification.Combined detection of IL-8 and CXCR2 mRNA may serve as a potential biomarker for the diagnosis and prognostic assessment of MDS.