The correlation and clinical significance of GART,RUVBL1 expression and stemness genes in colorectal cancer tis-sues

Xiong Zhiqiang; Liu Duxian; Fu Luyu; Zhang Xiaoya; Jiang Wen; Zhao Huihui

doi:10.3969/j.issn.1671-6450.2025.05.006

Abstract：Objective To investigate the expression of glycinamide ribonucleotide transformylase(GART)and RuvB like AAA ATPase 1(RUVBL1)in colorectal cancer(CRC)tissues and their correlation with sternness genes and clinical significance.Methods The clinical data of 96 patients with CRC diagnosed and treated in Nanjing Hospital Affilia-ted to Nanjing University of Traditional Chinese Medicine(Oncology Department of Nanjing Second Hospital)from Febru-ary 2019 to February 2021 were retrospectively collected.Real-time fluorescence quantitative PCR was used to detect the expression levels of GART mRNA,RUVBL1 mRNA and stemness gene[CD44,octamer binding transcription factor 4(OCT4),Nanog]mRNA in tissues;Pearson correlation was used to study the correlation between GART mRNA,RUVBL1 mRNA and stemness genes;The expression of GART and RUVBL1 protein was detected by immunohistochemistry;The effects of GART and RUVBL1 protein expression on the prognosis of CRC were statistically analyzed.Results Compared with the adjacent tissues,the expression of GART mRNA,RUVBL1 mRNA and sternness-related genes CD44 mRNA,OCT4 mRNA and Nanog mRNA in cancer tissues were higher(t/P=37.245/＜0.001,40.681/＜0.001,38.118/＜0.001,31.118/＜0.001,34.543/＜0.001);The expression of GART mRNA and RUVBL1 mRNA in CRC was positively correla-ted with the expression of CD44 mRNA,OCT4 mRNA and Nanog mRNA(r=0.712,0.730,0.639;0.661,0.641,0.742,all P＜0.001);The positive rates of GART and RUVBL1 protein in CRC tissues were 66.67％(64/96)and 64.58％(62/96),which were higher than those in adjacent normal tissues 625％(6/96)and 7.29％(7/96)(x2=75.631,68.434,all P＜0.001).The positive rates of GART and RUVBL1 protein in CRC tissues with TNM stage Ⅲ and lymph node metastasis were significantly increased(x2/P=10.371/0.001,5.486/0.019,9.623/0.002,5.002/0.025);The 3-year overall survival rates of GART positive group and RUVBL1 positive group were 51.56％(33/64)and 51.61％(32/62),respectively,which were lower than those of GART negative group and RUVBL1 negative group 81 25％(26/32)and 79.41％(27/34),the differ-ence was statistically significant(Log-rank x2=8.521,7.354,P=0.004,0.007).TNM stage Ⅲ and lymph node metastasis,high GART and high RUVBL1 were independent factors affecting the survival and prognosis of CRC[OR(95％CI)=1.326(1.083-1.622),1370(1.092-1.720),1.332(1.104-1.608),1.314(1.112-1.552)].Conclusion The expression of GART and RUVBL1 in CRC cancer tissues is increased,which is positively correlated with the expression of stemness-related genes,both of them are tumor markers for CRC prognosis evaluation.