The expression and clinical prognostic value of DDX5 and USP22 in hepatitis B-related hepatocellular carcinoma
Li Bo
Jiang Wen
Yu Jinhong
Zhang Yongchen
Wang Haiyu
Abstract：Objective To study the expression of DEAD-box helicase 5(DDX5)and ubiquitin specific peptidase 22(USP22)in hepatitis B virus-related hepatocellular carcinoma(HBV-HCC)tissues and their clinical prognostic signifi-cance.Methods The cancer tissues and adjacent tissues of 124 patients with HBV-HCC admitted to the Department of On-cology,Nanjing Hospital Affiliated to Nanjing University of Traditional Chinese Medicine from April 2020 to April 2021 were selected.Real-time fluorescence quantitative PCR(qPCR)and immunohistochemistry were used to detect the expres-sion of DDX5 and USP22 mRNA and protein in cancer tissues and adjacent tissues.Kaplan-Meier curve was used to analyze the effect of DDX5 and USP22 protein expression on the survival and prognosis of patients with HBV-HCC;Cox regression analysis was used to analyze the prognostic factors of HBV-HCC patients.Results The relative expression of DDX5 mRNA in cancer tissues of HBV-HCC patients was lower than that in adjacent tissues,and the relative expression of USP22 mRNA was higher than that in adjacent tissues(t/P=29.058/＜0.001,44.386/＜0.001);The positive rate of DDX5 protein in cancer tissues was lower than that in adjacent tissues(3226％ vs.8226％),and the positive rate of USP22 protein was higher than that in adjacent tissues(64.52％ vs.9.68％),the difference was statistically significant(x2/P=63.335/＜0.001,79.902/＜0.001);The positive rate of DDX5 protein in HBV-HCC tissues with tumor maximum diameter ≥ 5cm and CNLC stage Ⅱ-Ⅲ was lower than that with tumor maximum diameter＜5 cm and CNLC stage Ⅰ,and the positive rate of USP22 protein was higher than that with tumor maximum diameter＜5cm and CNLC stage Ⅰ(x2/P=8.712/0.003,9.501/0.002;12.113/＜0.001,9.983/0.002);The 3-year overall survival rate of DDX5 positive group was 82.50％(33/40),which was higher than 44.05％(37/84)of DDX5 negative group(Log rank x2=15.700,P＜0.001).The 3-year overall survival rate of the USP22 positive group was 45.00％(36/80),which was lower than that of the negative group(77 27％,34/44)(Log rank x2=11.640,P=0.001);CNLC stage Ⅱ～Ⅲ and USP22 positive were independent risk factors affecting the prognosis of HBV-HCC patients[HR(95％CI)=1.451(1.081-1.946),1.449(1.044-2.024)],DDX5 positive was an independent protective factor[HR(95％CI)=0.655(0.471-0.911)].Conclusion DDX5 expression is down-regulated and USP22 expression is up-regulated in HBV-HCC tissues.Both of them play a promoting role in the occurrence and progression of HBV-HCC and are markers for evaluating the prognosis of HBV-HCC patients.
Keywords：Hepatitis B virus associated liver cancerDEAD box helicase 5Ubiquitin specific peptidase 22Prog-nosis
Publication Date：2025-05-18
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 539-544 )