EPPK1,LPCAT1 expression in esophageal squamous cell carcinoma tissue and their clinical prognostic value
Yao Junjie
He Zhitao
Li Xiaoming
Xiang Zongjun
Li Lei
Jiang Chaoxia
Zhang Fan
Abstract：Objective To investigate the expression of Epiplakin 1(EPPK1),Lysophosphatidylcholinergic transam-inase 1(LPCAT1)in esophageal squamous cell carcinoma(ESCC)tissues,and their relationship with clinical pathological characteristics and prognosis.Methods One hundred and twelve ESCC patients admitted to the Department of Thoracic Surgery at the Guangyuan Central Hospital from May 2019 to May 2021 were selected as the research subjects.QPCR and immunohistochemistry were used to detect the mRNA and protein expression of EPPK1 and LPCAT1 in cancer tissues and adjacent tissues;Kaplan Meier curve analysis of the impact of EPPK1 and LPCAT1 protein expression on the survival prog-nosis of ESCC patients;Cox regression analysis of prognostic factors in ESCC patients.Results The relative expression levels of EPPK1 and LPCAT1 mRNA in cancer tissues of ESCC patients were higher than those in adjacent tissues(t/P=31.452/＜0.001,25.380/＜0.001);The positive rates of EPPK1 and LPCAT1 in ESCC cancer tissues were 64.29％(72/112)and 66.07％(74/112),respectively,which were higher than those in adjacent tissues(8.93％(10/112)and 12.50％(14/112),respectively(x2/P=136.899/＜0.001,129.328/＜0.001);The positive rates of EPPK1 and LPCAT1 proteins in ESCC cancer tissues with lymph node metastasis in TNM stage Ⅲ are higher than those in TNM stage Ⅰ-Ⅱ without lymph node metastasis(x2/P=28.910/＜0.001,18.500/＜0.001 20.931/＜0.001,11.784/＜0.001);The 3-year overall survival rate of the EPPK1 positive group was 43.06％(31/72),which was lower than the 85.00％(34/40)of the negative group(Log Rank x2=9.748,P=0.002).The 3-year overall survival rate of the LPCAT1 positive group was 43 24％(32/74),which was lower than the negative group's 86.84％(33/38)(Log Rank x2=10.110,P=0.001);TNM stage Ⅲ,lymph node metastasis,EPPK1 positivity,and LPCAT1 positivity were independent risk factors affecting the prognosis of ESCC patients[HR(95％CI)=1.730(1.434-2.099),1.531(1215-1.930),1.898(1.331-2.707),1.677(1.124-2.501)].Conclusion The elevated expres-sion of EPPK1 and LPCAT1 in esophageal cancer are associated with adverse clinical and pathological features,and are new tumor markers for evaluating the poor prognosis of ESCC.
Keywords：Esophageal squamous cell carcinomaEpiplakin 1Lysophosphatidylcholine acyltransferase 1Prognosis
Publication Date：2025-05-18
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 526-531 )