The levels and prognostic value of serum miR-10a and miR-21 in end-stage renal disease hemodialysis patients
Qian Rui
Huang Wenhui
Li Yingping
Lu Shouyan
Dong Liting
Jia Jingjing
Xue Rong
Abstract：Objective To investigate the expression levels of serum microRNA-10a(miR-10a)and microRNA-21(miR-21)in end-stage renal disease(ESRD)hemodialysis patients and their predictive value for prognosis.Methods One hundred and fifty ESRD patients who underwent regular haemodialysis treatment at the Blood Purification Centre,Gansu Provincial Hospital from October 2021 to October 2023 were selected as the study group,and 60 patients with chronic kid-ney disease stages 4-5 who required dialysis treatment for the first time in our hospital were randomly selected as the con-trol group.Total cholesterol(TC),triacylglycerol(TG),blood creatinine(SCr),urea nitrogen(BUN),uric acid(UA),eGFR,haemoglobin,C-reactive protein(CRP),blood calcium,and blood phosphorus were detected,and the expression levels of miR-10a and miR-21 were detected by real-time fluorescence quantitative polymerase chain reaction(RT-qPCR);the statistics of patients of the study group after 1 year of follow-up Survival and death,the predictive value of serum miR-10a and miR-21 expression levels on the prognosis of ESRD haemodialysis patients was analysed by using subject operating characteris-tic(ROC)curves,and logistic regression was used to analyse the factors affecting the prognosis of ESRD haemodialysis pa-tients.Results Compared with the control group,the study group had elevated levels of SCr,serum miR-10a,and miR-21 expression(t=18.059,7349,8.391,P＜0.001),and decreased levels of eGFR(t=28.867,P＜0.001).Compared with the survival subgroup,the death subgroup BUN,miR-10a and miR-21 expression levels were elevated(t/P=5.342/＜0.001,8.703/＜0.001,8273/＜0.001),and haemoglobin,eGFR levels were decreased(t/P=2.324/0.022,2.760/0.007);miR-10a,miR-21 alone predicted and both combination predicted the prognosis of SRD haemodialysis patients with AUCs of 0.872,0.826 and 0.944,respec-tively,and the combination of the two was superior to their respective individual predictive efficacy(Z/P=2.635/0.030,3 294/0.014);low eGFR,serum miR-10a expression level was high,and miR-21 expression level was high were all risk factors for prognostic mortality in ESRD haemodialysis patients[OR(95％CI)=1.409(1.100-1.804),1.364(1.078-1.726),1.395(1.164-1.671)].Conclusion The levels of miR-10a and miR-21 in the serum of ESRD hemodialysis patients are obviously upregulated,both of which are risk factors affecting the prognosis and death of ESRD hemodialysis patients.The combined prediction of the two has high predictive value for the prognosis of ESRD patients.
Keywords：End stage renal diseaseHemodialysismiR-10amiR-21Prognostic value
Publication Date：2025-04-18
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 468-472 )