Correlation analysis of serum SIRT6,SLC7A11 and type 2 diabetes mellitus combined with non-alcoholic fatty liver disease

Ren Lihong; Guo Xi; Shi Ligang; Zhang Hongying; Guo Weiyan

doi:10.3969/j.issn.1671-6450.2025.04.007

Abstract：Objective To investigate the correlation between serum silent information regulator factor 6(SIRT6),solute carrier family 7 member 11(SLC7A11),and type 2 diabetes mellitus(T2DM)combined with non-alcoholic fatty liver disease(NAFLD).Methods A total of 90 patients with T2DM complicated with NAFLD,who were admitted to the Depart-ment of Endocrinology at Harbin Second Hospital from January 2022 to March 2024,were enrolled in the NAFLD group.Another 90 T2DM patients without NAFLD were selected in a 1∶1 ratio as the non-NAFLD group.Serum SIRT6 and SLC7A11 levels were detected using enzyme-linked immunosorbent assay.Multivariate logistic regression was performed to analyze the influencing factors of T2DM complicated with NAFLD.Receiver operating characteristic(ROC)curves were plot-ted to evaluate the diagnostic efficiency of serum SIRT6 and SLC7A11 levels for T2DM complicated with NAFLD.Results Serum SIRT6 and SLC7A11 levels were significantly lower in the NAFLD group compared to the non-NAFLD group(t/P=7.892/＜0.001,7.643/＜0.001).The alkaline phosphatase(ALP),aspartate aminotransferase(AST),alanine aminotrans-ferase(ALT),and triglyceride(TG)levels were higher in the NAFLD group than in the non-NAFLD group(t/P=2.953/0.004,3.764/＜0.001,4.729/＜0.001,4.862/＜0.001).Body mass index(BMI),hypertension,ALP,AST,ALT,and TG were independ-ent risk factors for T2DM complicated with NAFLD,while high SIRT6 and SLC7A11 levels were independent protective fac-tors[OR(95％CI)=1279(1.044-1.569),2 342(1.096-5.005),1.026(1.001-1.052),1.038(1.003-1.074),1.026(1.001-1.052),2.524(1.307-4.874),0.782(0.692-0.882),0.987(0.981-0.994)].The area under the curve(AUC)for serum SIRT6,SLC7A11,and their combination for diagnosing T2DM complicated with NAFLD were 0.791,0.787,and 0.876,respectively.The com-bined diagnosis of serum SIRT6 and SLC7A11 for T2DM complicated with NAFLD showed an AUC greater than the indi-vidual diagnosis by SIRT6 and SLC7A11(Z/P=2.217/0.027,2.041/0.041).Conclusion The decrease in serum SIRT6 and SLC7A11 levels in T2DM patients is closely related to T2DM complicated with NAFLD.The combination of serum SIRT6 and SLC7A11 levels has high diagnostic efficiency for T2DM complicated with NAFLD.