Expression and clinical significance of interleukin-30 and autophagy-related gene 5 in psoriasis vulgaris
Yao Ming
Lu Yonghong
Chen Hao
Pei Baoqiang
Li Zaibing
Abstract：Objective To investigate the expression and clinical significance of serum interleukin-30(IL-30)and au-tophagy-related gene 5(ATG5)in psoriasis vulgaris(PV).Methods A total of 100 PVpatients(PV group)and 100 healthy controls(control group)were enrolled from January 2022 to August 2024 at the Department of Dermatology,Chengdu Sec-ond People's Hospital.Based on disease severity,PV patients were further divided into mild PV subgroup(27 cases),mod-erate PV subgroup(37 cases),and severe PV subgroup(36 cases).Serum levels of IL-30,ATG5,T helper 17(Th17)down-stream factors(IL-6,IL-17A),and regulatory T cell(Treg)downstream factors[IL-10,transforming growth factor-β1(TGF-β1)]were measured by ELISA.Peripheral blood proportions of Treg,Th17,and the Treg/Th17 ratio were assessed using flow cytometry.Pearson correlation analyses were conducted to explore relationships between serum IL-30,ATG5 levels and disease severity,Th17-and Treg-related factors,and the peripheral blood Th17/Treg ratio.The ROC curve was used to eval-uate the diagnostic performance of serum IL-30 and ATG5 for moderate to severe PV.Results Compared with the control group,the PV group showed significantly elevated serum IL-30,ATG5,IL-6,IL-17A,and peripheral Th17 levels and Thl7/Treg ratio,while IL-10,TGF-β1(t/P=10.276/＜0.001,10.621/＜0.001,20.729/＜0.001,29.252/＜0.001,17.046/＜0.001,16.972/＜0.001),and Treg proportions were decreased(t/P=18.426/＜0.001,28.000/＜0.001,12.506/＜0.001).Serum IL-30,ATG5,IL-6,IL-17A,and peripheral Th17 levels and Th17/Treg ratio progressively increased across mild,moderate,and severe PV sub-groups(F/P=176.054/＜0.001,201.964/＜0.001,172.705/＜0.001,192.062/＜0.001,213.010/＜0.001,256.673/＜0.001),whereas IL-10,TGF-β1,and Treg levels progressively decreased(F/P=105.130/＜0.001,211.495/＜0.001,123.180/＜0.001).Serum IL-30 and ATG5 levels were positively correlated with disease severity,IL-6,IL-17A,peripheral Th17 levels,and the Th17/Treg ra-tio(r/P=0.703/＜0.001,0.723/＜0.001,0.686/＜0.001,0.712/＜0.001,0.772/＜0.001,0.790/＜0.001,0.702/＜0.001,0.790/＜0.001,0.710/＜0.001,0.719/＜0.001),and negatively correlated with IL-10,TGF-β1,and Treg levels(r/P=-0.633/＜0.001,-0.678/＜0.001,-0.768/＜0.001,-0.767/＜0.001,-0.643/＜0.001,-0.707/＜0.001).The AUCs for serum IL-30,ATG5,and their com-bined assessment in evaluating moderate to severe PV were 0.799,0.784,and 0.876,respectively,with the combined AUC significantly outperforming each individual marker(Z/P=2.274/0.023,2.414/0.016).Conclusion Elevated serum IL-30 and ATG5 levels in PVpatients are closely associated with disease severity and Th17/Treg imbalance.The combined assessment of IL-30 and ATG5 levels provides high diagnostic efficacy for moderate to severe PV.
Keywords：Psoriasis vulgarisInterleukin-30Autophagy-related gene 5Severity of illnessT helper cells 17/T regulatory cells ratio
Publication Date：2025-03-18
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 354-358,366 )