Correlation between miR-222-3p and SLFN11 expressions in cervical cancer tumor tissues and clinicopathological fea-tures and prognosis
Zhang Peng
Zeng Xiaoyan
Zhou Xiaoli
Wang Liming
Zhang Yajun
Abstract：Objective To investigate the correlation between the expression of miR-222-3p and SLFN11 in cervical cancer tumor tissue and clinical pathological characteristics and prognosis.Methods A total of 103 patients with cervical cancer treated in the General Hospital of the Central Theater Command of the Chinese People's Liberation Army from January 2019 to June 2021 were divided into the cervical cancer group and 57 patients with benign cervical diseases as the control group.Real-time fluorescence quantitative polymerase chain reaction was used to detect the expression of miR-222-3p and SLFN11 in the two groups of patients;the differences in the expression of miR-222-3p and SLFN11 in tumor tissues of pa-tients with different clinical pathological characteristics of cervical cancer were analyzed;Correlation analysis was performed using Spearman rank correlation,poor prognostic efficacy of cervical cancer was analyzed using ROC curve analysis and compared using DeLong method,risk factors were analyzed using multivariate Cox regression analysis,and survival analysis was performed using K-M method and Log Rank comparison.Results The expression of miR-222-3p in tumor tissue of cervical cancer group was higher than that in adjacent tissues and control group,while the expression of SLFN11 in adja-cent tissues and control group was lower(F/P=945.371/＜0.001,226.816/＜0.001).The expression of miR-222-3p in patients with positive CTC,Ki-67 ≥ 70％,T stage T3-4,N stage N1,distant metastasis M1 and TNM stage Ⅲ-Ⅳ was higher than that in patients with negative CTC,Ki-67＜70％,T stage T1-2,N stage Nx-N0,no distant metastasis and TNM stageⅠ-Ⅱ(t/P=3.263/0.002,4.208/＜0.001,4.182/＜0.001,3.342/0.001,5.391/＜0.001,3.298/＜0.001),SLFN11 expression was lower than that in patients with negative CTC,Ki-67＜70％,T stage T1-2,N stage Nx-N0,no distant metastasis and TNM stageⅠ-Ⅱ(t/P=5.140/＜0.001,5.706/＜0.001,5.984/＜0.001,7.701/＜0.001,6.704/＜0.001,7.859/＜0.001)In the cervical cancer group,the expression of miR-222-3p in tumor tissue was positively correlated with CTC,Ki-67,T stage,N stage,distant me-tastasis,and TNM stage(rs/P=0.683/0.035,0.704/0.009,0.626/0.021,0.651/0.029,0.627/0.023,and 0.649/0.015),and the expres-sion of SLFN11 was negatively correlated with CTC,Ki-67,T stage,N stage,distant metastasis,and TNM stage(rs/P=-0.624/0.037,-0.651/0.025,-0.677/0.032,-0.632/0.019,-0.648/0.007,and-0.675/0.023).The AUCs of miR-222-3p,SLFN11 and their combination for predicting poor prognosis in cervical cancer patients were 0.674,0.652 and 0.883,respec-tively.The combination of miR-222-3p and SLFN11 was superior to their individual predictive efficacy(Z/P=8.143/＜0.001,10.561/＜0.001).CTC positivity,Ki-67 ≥ 70％,T stage T3-4,N stage N1,distant metastasis,TNM stage Ⅲ-Ⅳ,miR-222-3p≥1.18 and SLFN11 ≤ 0.65 were independent risk factors for poor prognosis of cervical cancer[HR(95％CI)=2.776(1.128-4.424),1.865(1.044-2.685),2.056(1.095-3.018),2.192(1.127-3.257),3.473(1.133-5.813),2.656(1.026-4.286),5.371(1.644-9.098),4.367(1.512-7.221)];the median survival of cervical cancer patients with miR-222-3p ≥ 1.18 and SLFN11 ≤0.65 was(25.19±4.36)months,which was lower than that of patients with miR-222-3 P＜1.18 or SLFN11＞0.65(30.68±5.21)months(Log Rankx2=9.046,P＜0.001).Conclusion The expression of miR-222-3p and SLFN11 in cervical cancer tumor tissue is closely related to clinical pathological characteristics,prognosis and survival.The combined detection of the two can signifi-cantly improve the value of poor prognosis assessment in cervical cancer.
Keywords：Cervical cancerMicroRNA-222-3pSLFN11Prognosis
Publication Date：2025-03-18
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 296-302 )