Expression and clinical value of miR-92a and NOC2L in ovarian cancer tumor tissue
Zeng Yafen
He Qunfang
Ruan Yuqing
Wang Jing
Wang Liming
Abstract：Objective To study the expression of miR-92a and NOC2-like nucleolar-associated transcriptional re-pressor(NOC2L)in ovarian cancer(OVC)tissues and their relationship with clinicopathological characteristics and progno-sis.Methods A total of 103 OVC patients treated in Wuhan Third Hospital from January 2019 to December 2021 were se-lected as the research subjects.Real-time fluorescence quantitative PCR was used to detect the mRNA expressions of miR-92a and NOC2L in cancer tissues and adjacent tissues.Receiver operating characteristic(ROC)curve was used to ana-lyze the predictive efficacy of miR-92a and NOC2L mRNA expressions in cancer tissues on the prognosis and death of OVC patients,and the DeLong method was used to compare the differences.Multivariate Logistic regression was used to analyze the influencing factors of the prognosis and death of OVC patients.The K-M method and Log Rank method were used to compare the differences in survival time in different miR-92a and NOC2L expressions.Results The relative expression lev-els of miR-92a and NOC2L mRNA in cancer tissues of OVC patients were higher than those in adjacent tissues(t/P=22.995/＜0.001,16.691/＜0.001).The mRNA expressions of miR-92a and NOC2L in cancer tissues of OVC patients with Shimi-zu-Silverberg grade 3,positive ascites cytology,lymph node metastasis N1,distant metastasis M1,and FIGO stage Ⅲ-Ⅳwere higher than those with Shimizu-Silverberg grade 1-2,negative ascites cytology,lymph node metastasis N0,distant me-tastasis M0,and FIGO stage Ⅰ-Ⅱ(miR-92a:t/P=2.020/0.046、2.635/0.009、1.994/0.049、2.981/0.004、2.807/0.006;NOC2L:t/P=3.483/0.001、3.729/＜0.001、3.183/0.002、6.269/＜0.001、4.318/＜0.001).The mRNA expression was positively correlated with Shimizu-Silverberg grade,ascites cytology,lymph node metastasis,distant metastasis,and FIGO stage(miR-92a:rs/P=0.678/0.024,0.715/0.007,0.699/0.015,0.742/0.003,0.687/0.029;NOC2L:rs/P=0.631/0.015,0.687/0.029,0.711/0.024,0.659/0.031,0.702/0.008).The areas under the curve(AUC)of miR-92a,NOC2L and their combination for predicting the prognosis and mortali-ty of OVC patients were 0.684,0.701 and 0.896,respectively.The combination of miR-92a and NOC2L was superior to the single predictive efficacy(Z/P=7.145/＜0.001,6.097/＜0.001).Multivariate Logistic regression analysis showed that miR-92a ≥ 1.45 and NOC2L≥0.86 were independent risk factors for prognosis and mortality in OVC patients[OR(95％CI)=5.013(1.399-7246),4581(1.175-6.628)].The median survival of OVC patients with miR-92a≥ 1.45 and NOC2L≥0.86 was lower than that of OVC patients with miR-92a＜1.45 or NOC2L＜0.86(Log Rankx2=14.026,P＜0.001).Conclusion The ex-pression of miR-92a and NOC2L is increased in cancer tissues of OVC patients.Both can provide objective evidence for the assessment of the condition and prognosis of OVC patients.When combined,the clinical value of death prediction in OVC patients can be significantly improved.
Keywords：Ovarian cancerMicroRNA-92aNOC2-like nucleolar-associated transcriptional repressorClinicopath-ological characteristicsPrognosticClinical value
Publication Date：2025-03-18
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 290-295 )