The relationship between miR-497-5p and FOXP4 expression in papillary thyroid carcinoma tissues with pathological parameters and prognosis
Xiang Hui
Jia Xiaobin
Ke Xianfeng
Wang Xiyu
Li Qiang
Abstract：Objective To investigate the relationship between the expression of micro RNA-497-5p(miR-497-5p)and forkhead box P4(FOXP4)in papillary thyroid carcinoma(PTC)tissues and their association with pathological parameters and prognosis.Methods A total of 153 PTC patients treated in the Breast and Thyroid Surgery Department of Shangluo Central Hospital from January 2017 to August 2019 were selected for this study.Quantitative real-time PCR was used to de-tect miR-497-5p and FOXP4 mRNA expression in PTC and adjacent non-cancerous tissues.The correlation between miR-497-5p and FOXP4 mRNA levels was analyzed using Pearson correlation.Kaplan-Meier survival analysis assessed the im-pact of miR-497-5p and FOXP4 expression on patient prognosis,while Cox regression was used to identify factors affecting PTC prognosis.Results The miR-497-5p expression was significantly lower,while FOXP4 mRNA expression was signifi-cantly higher in PTC tissues compared to adjacent non-cancerous tissues(t/P=35.062/＜0.001,38.007/＜0.001).A binding site was identified in the 3'-untranslated region of FOXP4 for miR-497-5p.Pearson correlation showed a negative correlation between miR-497-5p and FOXP4 mRNA levels in PTC tissues(r/P=-0.664/＜0.001).miR-497-5p levels were significantly lower,and FOXP4 mRNA levels higher in stages Ⅲ-ⅣB and in cases with lymph node metastasis compared to stages Ⅰ-Ⅱ and cases without lymph node metastasis(t/P=3.013/0.003,2.834/0.005,3.027/0.003,2.872/0.005).The five-year survival rate was 84.31％(129/153)among all PTC patients,with a significantly higher survival rate in the high miR-497-5p group(96.00％,72/75)compared to the low miR-497-5p group(73.08％,57/78)(Log-rank x2=15.118,P＜0.001).Conversely,the high FOXP4 mRNA group had a lower five-year survival rate(7123％,52/73)compared to the low FOXP4 mRNA group(9625％,77/80)(Log-rank x2=18.128,P＜0.001).Advanced TNM stage(Ⅲ-ⅣB),lymph node metastasis,and elevated FOXP4 mRNA were independent risk factors for poor prognosis in PTC patients[HR(95％CI)=3.835(1.373-10.715),3.061(1.106-8.472),and 4.841(1.601-14.633)],while elevated mriR-497-5p was an independent protective factor[HR(95％CI)=0.193(0.056-0.666)].Conclusion Low expression of miR-497-5p and high expression of FOXP4 mRNA in PTC tissues are associated with unfa-vorable pathological parameters and poor prognosis.
Keywords：Papillary thyroid carcinomaMicroRNA-497-5pForkhead box protein P4Prognosis
Publication Date：2025-02-17
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)