Correlation analysis between serum SIRT6,CHI3L1 expression and degree of liver fibrosis in patients with chronic hepatitis B
Liu Hong
Qian Xiaoxian
Li Xiang
Zhang Zhifeng
Chen Chunyan
Abstract：Objective To investigate the relationship between the expression levels of Sirtuin-6(SIRT6)and Chiti-nase-3-like protein 1(CHI3L1)in the serum of patients with chronic hepatitis B(CHB)and the severity of liver fibrosis.Methods A total of 124 CHB patients admitted to our hospital between March 2023 and April 2024 were included as the research subjects.According to the Scheuer system scoring results,they were assigned into a significant liver fibrosis group(Scheuer score ≥ 2)of 58 cases and a non-significant liver fibrosis group(Scheuer score＜2)of 66 cases.ELISA method was used to detect the levels of serum SIRT6 and CHI3L1.Multivariate logistic regression analysis was applied to determine the risk factors for the degree of liver fibrosis in CHB patients.The diagnostic value of serum SIRT6 and CHI3L1 for the degree of liver fibrosis in CHB patients was analyzed by plotting ROC curves.Results Compared with the non-sig-nificant liver fibrosis group,the significant liver fibrosis group showed a significant decrease in serum SIRT6 level and a sig-nificant increase in serum CHI3L1 level in CHB patients(t/P=6.719/＜0.001,7.546/＜0.001).The proportion of HBeAg posi-tive individuals and HBV-DNA level in the significant liver fibrosis group were higher than those in the non-significant liver fibrosis group(t/P=24.125/＜0.001,10246/＜0.001).CHI3L1 was an independent risk factor for the degree of liver fibrosis in CHB patients[OR(95％CI)=2.932(1.554-5.533)],while SIRT6 was a protective factor for the degree of liver fibrosis in CHB patients[OR(95％CI)=0586(0.435-0.789)].The AUC of serum SIRT6,CHI3L1 and value the combined diagnosis for the degree of liver fibrosis in CHB patients was 0.897,0.856,0.963,and the AUC of the combined diagnosis was better than that of the individual diagnosis(Z=2.888,3.676,P=0.004,0.000).Conclusion The serum SIRT6 level is decreased and CHI3L1 level is increased in CHB patients,they are related to the degree of liver fibrosis.The combination of the two has certain value in diagnosing the degree of liver fibrosis in CHB patients.
Keywords：Chronic hepatitis BDegree of liver fibrosisSirtuin-6Chitinase 3-like protein 1Correlation
Publication Date：2025-02-17
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)