The value of tumor tissue miR-199a combined with SNAI1 detection in colorectal cancer disease and prognosis assess-ment
Bian Yaogang
Shen Ye
Zhang Qiang
Wei Jun
Tang Bin
Shi Huisen
Abstract：Objective To investigate the value of miR-199a combined with SNAI1 detection in tumor tissues in the evaluation of colorectal cancer(CRC)disease and prognosis.Methods A total of 94 patients with CRC who were treated in the Department of General Surgery of Fengxian District Central Hospital,Shanghai from December 2018 to December 2022 were selected as the CRC group and 51 patients with benign colorectal diseases were selected as the control group.Real-time fluorescence quantitative PCR was used to detect the expression of miR-199a and SNAI1 in the two groups.Spearman rank correlation analysis was used to analyze the correlation between the expression of miR-199a and SNAI1 in CRC tumor tissues and the clinical pathological characteristics of CRC patients.Kaplan-Meier survival model was used to analyze the effect of miR-199a and SNAI1 expression on the survival and prognosis of CRC patients.Multivariate Cox regression model was used to analyze the influencing factors of CRC patients'prognosis.Results The relative expression levels of miR-199a and SNAI1 in tumor tissues of CRC group were higher than those in control group(t/P=15.129/＜0.001,12.476/＜0.001).The expression levels of miR-199a and SNAI1 in tumor tissues of CRC patients with histological grade 3,T stage T3-T4,lymph node metastasis N2,distant metastasis,and TNM stage Ⅲ-Ⅳ were higher than those with histological grade 1-2,T stage T1-T2,lymph node metastasis N0-N1,no distant metastasis,and TNM stage Ⅰ-Ⅱ(miR-199a:t/P=2.002/0.048、2.095/0.039、1.995/0.049、2.003/0.048、2.933/0.004;SNAI1:t/P=2.595/0.01 1、3.634/＜0.001、3.871/＜0.001、4.975/＜0.001、7.077/＜0.001);The miR-199a and SNAI1 expressions in tumor tissues of CRC patients were positively correlated with histological grade,T stage,lymph node metastasis,distant metastasis,and TNM stage(miR-199a:r/P=0.643/0.038,0.699/0.012,0.612/0.015,0.679/0.011,0.654/0.019;SNAIl:r/P=0.611/0.043,0.637/0.026,0.644/0.017,0.656/0.009,0.671/0.004);The median survival of CRC pa-tients with miR-199 a ≥ 1.68 and SNAI1 ≥ 0.96 was significantly lower than that of other patients(miR-199a＜1.68 or SNAI1＜0.96)(median survival 26.48±3.72 months vs.33.81±5.59 months,Log Rank=19.141,P＜0.05).The survival rate of CRC pa-tients with miR-199a≥ 1.68 and SNAI1≥0.96 was significantly lower than that of other patients(miR-199a＜1.68 or SNAI1＜0.96)(3625％vs.51.17％,x2=9.435,P＜0.001).Histological grade 3,T stage T3 to T4,lymph node metastasis N2,distant metastasis,TNM stage Ⅲ to Ⅳ,elevated miR-199a,and elevated SNAI1 were independent risk factors for poor prognosis in CRC patients[HR(95％CI)=1.842(1278-2.407),2.004(1.129-2.879),2.502(1.114-3.891),3.105(1.077-5.133),2.779(1.092-4.465),4.586(1.566-7.607),4.315(1.431-7.198)].Conclusion The expression of miR-199a and SNAI1 in tumor tissues of CRC patients was significantly increased,which was associated with clinicopathological characteristics,prognosis and survival,and could be evidence at the gene level for colorectal cancer disease and prognosis evaluation.The combined detection of the two could significantly improve the clinical value in the evaluation of poor prognosis of colorectal cancer.
Keywords：Colorectal cancerMicro RNA-199aSNAI1PrognosisClinical value
Publication Date：2025-02-17
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)