The expression of serum kisspeptin and FOXO3 in patients with polycystic ovary syndrome and their prognostic sig-nificance for the disease
Li Yan
Wang Yunling
Ren Xuehui
Chang Ying
Wei Yanhu
Abstract：Objective To detect the expression of serum kisspeptin and forkhead box O subtype 3(FOXO3)in pa-tients with polycystic ovary syndrome(POS),and analyze the prognostic value of kisspeptin and FOXO3 expression in polycystic ovary syndrome.Methods From March 2021 to March 2024,94 patients with polycystic ovary syndrome who visited to the Gynecology Department of Yan'an People's Hospital were selected as the study group.According to the prog-nosis of the patients after 3 months of treatment,they were assigned into a poor prognosis subgroup(38 cases)and a good prognosis subgroup(56 cases).Additionally,60 healthy women of childbearing age who underwent physical checkup at our hospital were included as the healthy control group.ELISA method was applied to detect serum kisspeptin.RT-qPCR meth-od was applied to detect serum FOXO3.Multivariate logistic regression was applied to analyze the influencing factors of poor prognosis in patients.Receiver operating characteristic(ROC)curve analysis of serum kisspeptin and FOXO3 for pre-dicting poor prognosis in patients with polycystic ovary syndrome.Results The serum level of kisspeptin in the study group was higher than that in the healthy control group,and the level of FOXO3 was lower than that in the healthy control group(t/P=9.388/＜0.001,10.087/＜0.001).The levels of luteinizing hormone and kisspeptin in the poor prognosis subgroup were higher than those in the good prognosis subgroup,and FOXO3 was lower than that in the good prognosis subgroup(t/P=4.774/＜0.001,7.147/＜0.001,8.269/＜0.001).Elevated levels of luteinizing hormone and kisspeptin were independent risk factors for poor prognosis in patients with polycystic ovary syndrome[OR(95％CI)=2.455(1.402-4.300),2.174(1.432-3.300)],while elevated level of FOXO3 was a independent protective factor[OR(95％CI)=0.142(0.067-0.301)].The AUC of predicting poor prognosis in patients with polycystic ovary syndrome based on seruim levels of kisspeptin and FOXO3,as well as their combined AUC,were 0.791,0.792,and 0.906,respectively.The AUC of combined was greater than the AUC predicted by serum levels of kisspeptin and FOXO3 alone(Z/P=2.060/0.039,2.544/0.011).Conclusion Patients with poly-cystic ovary syndrome have higher serum level of kisspeptin and lower level of FOXO3.Elevated levels of serum kispeptin and decreased levels ofFOXO3 are factors affecting poor prognosis in patients.The combined detection of serum kisspep-tin and FOXO3 has certain significance for the prognosis of patients with polycystic ovary syndrome.
Keywords：Polycystic ovary syndromeKisspeptinForkhead box O subtype 3Prediction of prognosis
Publication Date：2025-01-17
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)