Effect of IncRNA SOX21-AS1 on H2O2-induced cardiomyocyte injury by regulating miR-7-5p expression
Wang Hua
Xin Xingchang
Liu Tiantian
Abstract：Objective To study whether long non-coding RNA(lncRNA)SOX21 antisense RNA1(SOX21-AS1)regulates H2O2-induced cardiomyocyte damage by targeting miR-7-5p.Methods From July 2022 to February 2023,the ex-periment was carried out in Qinghai Provincial People's Hospital.The H9C2 cells were treated with H2O2 to mimic an oxida-tive stress injury model.H9C2 cells was divided into Con group,H2O2 group,H2O2+si-NC group,H2O2+si-SOX21-AS1 group,H2O2+miR-NC group,H2O2+miR-7-5p group,H2O2+si-SOX21-AS1+anti-miR-NC group,H2O2+si-SOX21-AS1+anti-miR-7-5p group.Expression of SOX21-AS1 and miR-7-5p was detected using RT-qPCR The viability and apop-tosis of H9C2 were calculated using CCK-8 method and flow cytometry.A commercial kit was used to measure the activity of SOD and the content of malondialdehyde(MDA).Dual luciferase reporter experiment was used verify the targeting rela-tionship between SOX21-AS1 and miR-7-5p.Results After H2O2 treatment,cell viability,miR-7-5p expression and SOD ac-tivity of H9C2 were significantly reduced(P＜0.05),while apoptosis rate,SOX21-AS1 expression and MDA content were significantly increased(P＜0.05).Interference with SOX21-AS1 or miR-7-5p overexpression can promote H9C2 cell survival(P＜0.05),increase SOD activity(P＜0.05),reduce MDA content(P＜0.05),as well as inhibit cell apoptosis(P＜0.05).miR-7-5p is the target gene of SOX21-AS1.miR-7-5p inhibition can reverse the protective effect of interference SOX21-AS1 on H2O2-induced H9C2 cells(P＜0.05).Conclusion Interfering with SOX21-AS1 can reduce H2O2-induced cardiomyocyte in-jury by up-regulating miR-7-5p expression.
Keywords：H9C2 cellsSOX21-antisense RNA1Micro RNA-7-5pHydrogen peroxidCardiomyocytesOxidative stress
Publication Date：2024-12-18
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 1501-1506 )