Study on the relationship between serum VASH-1,HOTAIR expression and diabetes retinopathy
Ma Lei
Lin Yan
He Shenglin
Li Hui
Zhao Yang
Abstract：Objective To investigate the relationship between serum Vasohibin-1(VASH-1)protein,homeobox transcript antisense RNA(HOTAIR)expression and diabetic retinopathy(DR).Methods According to the ratio of 1:1:1,60 patients with PDR(PDR subgroup),60 patients with non-PDR(NPDR subgroup)and 60 patients with non-DR(NDR sub-group)admitted to the Department of Endocrinology in Shaanxi Provincial People's Hospital from January 2021 to January 2023 were selected as the T2DM group,and 90 healthy subjects were selected as the healthy control group,the expression of serum VASH-1 and HOTAIR was detected by enzyme-linked immunosorbent assay and real-time fluorescence quantita-tive PCR;Spearman method was used to analyze the correlation between serum VASH-1,HOTAIR expression and blood glucose parameters in T2DM patients;Multivariate disordered Logistic regression was used to analyze the factors of DR in patients with T2DM;ROC curve was drawn to evaluate the predictive value of serum VASH-1 and HOTAIR expression for DR and PDR.Results Compared with the healthy control group,the expression of serum VASH-1 and HOTAIR in the T2DM group increased(t/P=14.646/＜0.001,14203/＜0.001).The expression of serum VASH-1 and HOTAIR in NDR sub-group,NPDR subgroup and PDR subgroup increased in turn(F/P=11.728/＜0.001,43.850/＜0.001).The expression of serum VASH-1 and HOTAIR in T2DM patients was positively correlated with fasting blood glucose and glycosylated hemoglobin(HbA1c)(r/P=0210/0.005,0.458/＜0.001,0220/0.003,0.482/＜0.001).The independent risk factors for the occurrence and devel-opment of DR in T2DM patients were long course of T2DM,high HbA1c,high VASH-1 and high HOTAIR[OR(95％CI)=3.108(2.110-4.579)/2.011(1.487-2.719),6.634(2.761-15.940)/3.643(1.719-7.719),1.996(1.427-2.792)/1.587(1.196-2.104),2.600(1.683-4.015)/1.686(1201-2 366)].The AUC of DR and PDR predicted by the combination of the two was 0.869 and 0.886,respectively,which were higher than the AUC predicted by serum VASH-1 and HOTAIR expression alone,which were 0.803,0.785,and 0.787,0.778,respectively(Z/P=2.986/0.003,3230/0.001,3.581/＜0.001,3.837/＜0.001).Conclusion Elevated serum VASH-1 and HOTAIR expression are independent risk factors for the occurrence and development of DR in T2DM patients.Serum VASH-1 combined with HOTAIR expression has a high value in predicting DR and PDR.
Keywords：Diabetic retinopathyType 2 diabetes mellitusVasohibin-1Homeobox transcript antisense RNACor-relation
Publication Date：2024-12-18
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 1478-1483 )