Correlation between serum LncRNA CCHE1,TCF21 and myocardial ischemia in patients with coronary heart dis-ease complicated with hypertension

Wu Wenyu; Gao Gangli; Yang Liguo; Yao Jianqiang; Du Xiaoyan

doi:10.3969/j.issn.1671-6450.2024.12.004

Abstract：Objective To investigate the correlation between serum long non-coding RNA cervical cancer high ex-pression 1(LncRNA CCHE1),transcription factor 21(TCF21)and myocardial ischemia in patients with coronary heart disease(CHD)complicated with hypertension.Methods A total of 110 CHD patients with hypertension admitted to the Cardiovas-cular Department of the Yulin First Hospital from December 2020 to December 2023 were selected as the hypertensive group and 110 CHD patients without hypertension as the non-hypertensive group.According to the occurrence of myocardial is-chemia,the hypertension group was divided into 79 cases of myocardial ischemia subgroup and 31 cases of non-myocardial ischemia subgroup.The expression levels of serum LncRNA CCHE1 and TCF21 were determined by real-time fluorescence quantitative PCR;Pearson correlation analysis was used to analyze the correlation between serum LncRNA CCHE1,TCF21 expression levels and total myocardial ischemia burden(TIB);Multivariate logistic regression analysis was used to analyze the influencing factors of myocardial ischemia in CHD patients with hypertension;The receiver operating characteristic(ROC)curve was used to evaluate the diagnostic value of serum LncRNA CCHE1 and TCF21 expression levels for myocar-dial ischemia in CHD patients with hypertension.Results The expression level of serum LncRNA CCHE1 in the hyperten-sion group was higher than that in the non-hypertension group,and the expression level of serum TCF21 was lower than that in the non-hypertension group(t/P=19.133/＜0.001,17259/＜0.001);BMI,systolic blood pressure,proportion of three-vessel disease,serum Hcy level,TIB,serum LncRNA CCHE1 expression level in the myocardial ischemia subgroup were higher than those in the non-myocardial ischemia subgroup,and serum TCF21 expression level was lower than that in the non-myocardial ischemia subgroup(t/P=3.524/0.001,2.705/0.008,12.265/0.002,5.280/＜0.001,24.638/＜0.001,15.994/＜0.001,9280/＜0.001);Pearson correlation analysis showed that the expression level of serum LncRNA CCHE1 in CHD patients with hypertension was positively correlated with TIB(r=0.536,P＜0.001),and the expression level of serum TCF21 was negative-ly correlated with TIB(r=-0.508,P＜0.001);Multivariate Logistic regression analysis showed that three-vessel disease and high expression level of LncRNA CCHE1 were independent risk,factors for myocardial ischemia in CHD patients with hyper-tension[OR(95％CI)=3.465(1.534-7.833),1.743(1.135-2.678)],and high expression level of TCF21 was an independent pro-tective factor[OR(95％CI)=0.528(0312-0.895)];The area under the curve(AUC)of serum LncRNA CCHE1,TCF21 expres-sion levels and their combination in the diagnosis of myocardial ischemia in CHD patients with hypertension were 0.778,0.760 and 0.896,respectively.The AUC of the combination of the two was greater than that of serum LncRNA CCHE1 and TCF21 expression levels alone in the diagnosis of myocardial ischemia in CHD patients with hypertension(Z=2.636,3.088,P=0.002,＜0.001).Conclusion The increased expression of serum LncRNA CCHE1 and the decreased expression of TCF21 in CHD pa-tients with hypertension are related to myocardial ischemia.The combination of the two has a high value in the diagnosis of myocardial deficiency risk.