The correlation between serum NRG1,Klotho levels with EEG parameters,prognosis in elderly Alzheimer's disease patients
Liu Zhe
Xu Yan
Ma Xuan
Pan Jianping
Tang Wenyu
Abstract：Objective To investigate the correlation between serum Neuregulin 1(NRG1),Klotho with electroen-cephalogram parameters and prognosis in elderly patients with Alzheimer's disease.Methods A total of 192 elderly Alzhei-mer's disease patients who treated in the Department of Neurology Xi'an International Medical Center Hospital from January 2021 to December 2023 were included as the disease group,and were separated into a poor prognosis subgroup(87 cases)and a good prognosis subgroup(105 cases)according to their prognosis.In addition,82 healthy examinees were included as the healthy control group.Electroencephalogram detection was carried out,and(δ+θ)/(α+β)value was calculated.ELISA kit was applied to detect serum NRG1 and Klotho.The Mini Mental State Examination(MMSE)was applied to evaluate the cognitive function of AD patients,and grouped into asymptomatic subgroup(47 cases),mild subgroup(66 cases),moderate subgroup(70 cases),and severe subgroup(9 cases)based on the scores.General clinical data of the disease group were collected.Spearman was applied to analyze the correlation between various indicators.Logistic regression was applied to an-alyze the factors influencing the prognosis of Alzheimer's disease patients.ROC was applied to analyze the prognostic value of serum NRG1 and Klotho in Alzheimer's disease patients.Results The levels of NRG1 and Klotho in the disease group were lower than those in the healthy control group,and the(δ+θ)/(α+β)value was higher than that in the healthy control group(t/P=11.822/＜0.001,18.466/＜0.001,17.806/＜0.001).The trends of NRG1,Klotho,and MMSE levels showed asymp-tomatic subgroup＞mild subgroup＞moderate subgroup＞severe subgroup,and the trend of(δ+θ)/(α+β)value showed asymptomatic subgroup＜mild subgroup＜moderate subgroup＜severe subgroup(F/P=30.523/＜0.001,58.508/＜0.001,411.618/＜0.001,69.750＜0.001).NRG1 and Klotho are positively correlated with MMSE,while MMSE is negatively correlated with(δ+θ)/(α+β)(r/P=0.422/＜0.001,0.439/＜0.001,-0.418/＜0.001).The high-density lipoprotein cholesterol(HDL-C),NRG1,Klotho,MMSE,and proportion of daily exercise in the poor prognosis subgroup were lower than those in the good prognosis subgroup,while homocysteine(Hcy)and(δ+θ)/(α+β)value were higher than those in the good prog-nosis subgroup(t/x2/P=3.979/＜0.001,7.052/＜0.001,8.769/＜0.001,4.016/＜0.001,11305/0.001,5.936/＜0.001,9237/＜0.001).High Hey and(δ+θ)/(α+β)were risk factors for the prognosis of AD patients,,while high NRG1,Klotho,and MMSE were protective factors for the prognosis of AD patients[OR(95％CI)=2266(1.493-3.440),1.692(1.134-2.524),0.315(0.228-0.434),0384(0285-0518),0.401(0269-0.598)].The AUC of serum NRG1,Klotho,and their combination in predicting the prognosis of AD patients were 0.751,0.783,and 0.901,respectively.The combination of the two was superior to their indi-vidual predictive efficacy(Z=4.031,3.985,P＜0.001 for all).Conclusion The expression of serum NRG1 and Klotho is low in elderly Alzheimer's disease patients,and their expression levels are correlated with EEG parameters and prognosis.
Keywords：Alzheimer's diseaseNeuregulin 1KlothoElectroencephalogramPrognosisElderly
Publication Date：2024-11-18
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 1341-1345,1351 )