Correlation between serum LncRNA-MALAT1,Ang-2 and concurrent acute gastrointestinal injury in patients with severe acute pancreatitis

Ma Jiaju; Zou Han; Yan Qian; Li Yuzhuo; Pu Qinhua

doi:10.3969/j.issn.1671-6450.2024.10.013

Abstract：Objective To investigate the correlation between serum long non-coding RNA-metastasis-associated lung adenocarcinoma transcript 1(LncRNA-MALAT1),angiopoietin-2(Ang-2)and acute gastrointestinal injuries(AGI)in patients with severe acute pancreatitis(SAP)with complications.Methods Seventy-eight patients with mild AP admitted to the Department of Intensive Care Medicine of the Suzhou Ninth People's Hospital Affiliated to Suzhou University were se-lected as the mild AP group,78 patients with moderate SAP were selected as the moderate SAP group,and 145 patients with SAP were selected as the SAP group,and the patients with SAP were divided into the AGI subgroup of 83 and the non-AGI subgroup of 62 based on the presence or absence of concomitant AGI.Real-time fluorescence quantitative PCR was used to detect serum LncRNA-MALAT1 levels,and enzyme-linked immunosorbent assay was used to detect serum Ang-2 levels;multifactorial Logistic regression was used to analyze the influencing factors of SAP patients with concurrent AGI;and receiver operating characteristic(ROC)curves were used to evaluate the predictive value of serum LncRNA-MAL-AT1 and Ang-2 levels on SAP patients'predictive value of concurrent AGI.Results Serum LncRNA-MALAT1 and Ang-2 levels were sequentially increased in the mild AP,moderate SAP,and SAP groups(F/P=659.197/＜0.001,682.682/＜0.001);The complication rate of AGI in 145 SAP patients was 57.24％(83/145),and the proportion of multiple organ dysfunction syndrome(MODS),acute physiology and chronic health evaluation Ⅱ(APACHEⅡ)scores,duration of ICU stay,and serum D-lactate,LncRNA-MALAT1,and Ang-2 levels were higher in the SAP patients in the AGI subgroup than in the non-AGI subgroup(x2/t/P=19273/＜0.001,5.052/＜0.001,5.308/＜0.001,4.085/＜0.001,6.864/＜0.001,6.824/＜0.001);The results of multifactorial Logistic regression showed that high MODS,high APACHE Ⅱ score,high D-lactate,high LncRNA-MALAT1,and high Ang-2 were the independent risk factors for the complication of AGI in patients with SAP[OR(95％CI)=4.496(1.335-15.138),1331(1.142-1.551),1.070(1.020-1.123),1.101(1.055-1.148),1.571(1239-1.994)];the AUCs of serum Ln-cRNA-MALAT1 and Ang-2 levels and the combination of the two for predicting concurrent AGI in SAP patients were 0.790,0.785,and 0.895,respectively,and the AUCs of the combination of the two were greater than the AUCs predicted by serum LncRNA-MALAT1 and Ang-2 levels alone(Z/P=3.143/0.002,3.650/＜0.001).Conclusion Elevated serum LncRNA-MALATl and Ang-2 levels were independently associated with concurrent AGI in SAP patients,and serumLncRNA-MAL-AT1 combined with Ang-2 levels were of high value in predicting concurrent AGI in SAP patients.