Correlation of serum LncRNA Dlx6os1 and LncRNA TUG1 levels with renal injury and prognosis in elderly patients with diabetic nephropathy
Zhao Liaoliao
Cao Li
Chang Luyuan
Liu Yuyu
Si Yafang
Abstract：Objective To investigate the correlation between serum long non-coding RNA(LncRNA)distal-less homeobox6 antisense RNA 1(Dlx6os1),LncRNA taurine up-regulated gene 1(TUG1)levels and renal injury and prognosis in elderly diabetic nephropathy(DN)patients.Methods Two hundred and one elderly DN patients admitted to the Depart-ment of Nephrology,Yulin Hospital,the First Affiliated Hospital of Xi'an Jiaotong University from January 2019 to January 2021 were selected as the DN group,112 patients with pure T2DM during the same period as the pure T2DM group,and 105 healthy elderly people with physical examination during the same period as the control group,and the elderly DN pa-tients were divided into a poor prognosis subgroup(61 cases)according to the 3-year prognosis and a good prognosis subgroup(140 cases).The levels of serum LncRNA Dlx6os1,LncRNA TUG1 and renal injury indicators[estimated glomeru-lar filtration rate(eGFR),urinary albumin creatinine ratio(UACR)]were detected;the correlation between the levels of serum LncRNA Dlx6os1 and LncRNA TUG1 and renal injury of the elderly patients with DN was analyzed using Spearman's corre-lation coefficient;a multifactorial unconditional Logistic regression model to analyze the influence factors with the elderly DN patients'prognosis as the dependent variable,a multifactorial unconditional Logistic regression model was established to an-alyze the influencing factors,and the predictive value of serum LncRNA Dlx6os1 and LncRNA TUG1 levels was evaluated by plotting the receiver operating characteristics(ROC)curves.Results Serum LncRNA Dlx6os1 and UACR levels were se-quentially increased and serum LncRNA TUG1 and eGFR were sequentially decreased in the control,T2DM-only,and DN groups(F/P=870.484/＜0.001,566.671/＜0.001,271.117/＜0.001,196.722/＜0.001);Spearman's correlation showed that serum LncRNA Dlx6os1 levels were negatively correlated with eGFR and positively correlated with UACR in elderly DN patients(r/P=-0.816/＜0.001,0.809/＜0.001),and serum LncRNA TUG1 levels were positively correlated with eGFR and negatively cor-related with UACR(r/P=0.832/＜0.001,-0.806/＜0.001);Follow-up as of January 2024,the incidence of poor prognosis in 201 cases of elderly DN patients was 30.35％(61/201);multifactorial unconditional logistic regression showed that chronic kidney disease stage 4,high UACR,and high LncRNA Dlx6os1 were the independent risk factors for poor prognosis in eld-erly DN patients[OR(95％CI)=5.758(1.480-22.401),1.013(1.005-1.022),1.426(1201-1.693)],and high eGFR and high Ln-cRNA TUG1 were the independent protective factors[OR(95％CI)=0.958(0.939-0.978),0.418(0254-0.689)];ROC curves showed that the AUCs of serum LncRNA Dlx6os1,LncRNA TUG1,and the combination of the two for predicting poor prog-nosis in elderly DN patients were 0.774,0.777,and 0.852,respectively,and the AUCs for the combination of the two were the largest(Z/P=2930/0.003,3335/0.001).Conclusion Elevated serum LncRNA Dlx6os1 levels and reduced LncRNA TUG1 levels in elderly DN patients are associated with increased risk of aggravated renal injury and poor prognosis,and serum LncRNA Dlx6os1 in combination with LncRNA TUG1 levels are of high value for predicting poor prognosis in elderly DN patients.
Keywords：Diabetic nephropathyLong non-coding RNADistal-less homeobox6 antisense RNA 1Taurine up-regulated gene 1Kidney injuryPrognosisElderly
Publication Date：2024-08-18
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 934-939 )