The expression of FZD7 and GAL-8 in intrahepatic cholangiocarcinoma and their relationship with clinical pathologi-cal features and prognosis
Ma Yan'e
He Shuang
Liu Dongmei
Su Huyan
Wang Qianru
Hao Guangjun
Abstract：Objective To analyze the expression of Frizzled homolog protein 7(FZD7)and galactin 8(GAL-8)in in-trahepatic cholangiocarcinoma(ICC),and their relationship with clinical pathological features and prognosis.Methods Eighty-four ICC patients who visited Department of Oncology Yulin First Hospital from April2017 to April2020 were select-ed.Immunohistochemical methods were used to detect the expression of FZD7 and GAL-8 proteins in tissues.The relation-ship between FZD7 and GAL-8 were conducted by Spearman correlation analysis.Kaplan-Meier method was used to ana-lyze the differences in survival prognosis among ICC patients with different FZD7 and GAL-8 expressions.The prognostic factors for ICC were analyzed by Coxregression analysis.Results The positive rates of FZD7 and GAL-8 in ICC cancer tissue were 73.81%(62/84)and 71.43%(60/84),respectively,which were higher than those in adjacent cancer tissue by 4.76%(4/84)and 7.14%(6/84)(χ2=83.950,72.770,all P<0.001).FZD7 was positively correlated with GAL-8 expression in ICC(rs=0.745,P<0.001).The positive rates of FZD7 and GAL-8 were higher in ICCcancer tissues with low differentiation degree,lymph node metastasis and TNM stage Ⅲ(FZD7:χ2/P=8.221/<0.001,6.097/0.014,13.014/<0.001;GAL-8:χ2/P= 7.207/0.007,5.555/0.018,11.760/0.001).The 3-year overall survival rate of the FZD7 positive group was 32.26%(20/62),which was lower than the negative group's 68.19%(15/22)(Log rank χ2=8.723,P=0.003).The 3-year overall survival rate of the GAL-8 positive group was 28.33%(17/60),which was lower than the GAL-8 negative group's 75.30%(18/24)(Log rank χ2=24.310,P<0.001).TNM stageⅢ,low differentiation degree,lymph node metastasis,FZD7 positivity,and GAL-8 positivity were independent risk factors affecting the prognosis of ICC patients[HR(95%CI)=1.614(1.215-2.145),1.516(1.219-1.884),1.916(1.315-2.791),1.826(1.222-2.729),1.737(1.237-2.438)].Conclusion FZD7 and GAL-8 are upregulated in ICC,both of which are involved in the progression of ICC tumors and serve as tumor markers for evaluating the prognosis of ICC patients.
Keywords：Intrahepatic cholangiocarcinomaFrizzled homolog protein 7Galactin 8Prognosis
Publication Date：2024-05-18
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 590-594 )