Clinical value of miR-200c combined with MORC2 detection in the evaluation of the condition and prognosis of ovarian cancer
Hong Jia
Huang Jinling
Yang Qiongqiong
Wang Jing
Li Jing
Abstract：Objective To study the clinical value of micrornas 200c(miR-200c)combined with MORC family CW type zinc finger protein 2(MORC2)detection in the assessment of ovarian cancer disease and prognosis.Methods A total of 103 patients with ovarian cancer(ovarian cancer group)and 60 patients with benign ovarian diseases(control group)di-agnosed and treated in the Obstetrics and Gynecology Department of Renmin Hospital of Wuhan University from January 2017 to December 2019 were selected as the study objects.Real-time fluorescence quantitative PCR was used to detect the expression of miR-200c and MORC2 and analyze their relationship with clinicopathological factors.Receiver Operating char-acteristic(ROC)curve analysis of the efficacy of miR-200c combined with MORC2 in predicting 2-year poor prognosis of ovarian cancer.Multivariate Logistic regression analysis of risk factors of death in ovarian cancer patients.Kaplan-Meier method was used to analyze the relationship between miR-200c and MORC2 expression and survival.Results The expres-sions of miR-200c and MORC2 in tumor tissues of ovarian cancer group were higher than those in adjacent tissues and control group(F=1926.617,1832.415,P＜0.001).The expressions of miR-200c and MORC2 were higher in patients with low differentiation,malignant ascites,maximum tumor diameter≥5 cm,lymph node metastasis,distant metastasis,and stage Ⅲto Ⅳovarian cancer than those with medium-high differentiation,no malignant ascites,and maximumtumor diameter＜5 cm,no lymph node metastasis,no distant metastasis and stageⅠtoⅡpatients(t=14.067,12.451,12.871,11.523,16.298,18.351,11.745,10.893,10.462,9.893,14.617,13.269,P＜0.001).The AUC of miR-200c,MORC2 and their combination in predicting 2-year poor prognosis of ovarian cancer patients was 0.786,0.792 and 0.901,respectively,and the combined efficacy of miR-200C was superior to that of miR-200C and MORC2 alone(Z=8.239,8.025,P＜0.001).miR-200c≥0.78,MORC2≥0.65,poorly differentiated,malignant ascites,maximum tumor diameter≥5 cm,lymph node metastasis,distant metastasis,and FIGO stageⅢ to Ⅳ were independent risk factors for ovarian cancer death[OR(95%CI)=5.323(1.812-8.834),4.802(1.141-8.463),2.065(1.068-3.062),2.252(1.145-3.359),2.140(1.216-3.064),2.012(1.005-3.019),2.522(1.625-3.419),2.186(1.134-3.238)].The median survival time of ovarian cancer patients with miR-200c≥0.78 and MORC2≥0.65 was significantly lower than that of other patients(miR-200c＜0.78 or MORC2＜0.65)(Log-Rank χ2 =16.371,P＜0.05).Conclusion The expression of miR-200c and MORC2 is significantly increased in patients with ovarian cancer,which has important clinical value in the as-sessment of ovarian cancer disease and prognosis.The combined detection of miR-200C and MORC2 can significantly im-prove the sensitivity and specificity in predicting the poor prognosis of ovarian cancer.
Keywords：Ovarian cancerMicro RNA-200cMORC family CW type zinc finger protein 2Clinical valuePrognosis
Publication Date：2024-04-18
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 407-411 )