Expression and clinical significance of SIRT5 and CRIP1 in hepatocellular liver cancer tissues

Han Lu; Ji Aifang; Wei Zibai; Li Yanqi; Wang Biao

doi:10.3969/j.issn.1671-6450.2024.04.002

Abstract：Objective To investigate the expression and clinical significance of sirtiun 5(SIRT5)and cysteine rich protein 1(CRIP1)in hepatocellular carcinoma(HCC)tissues.Methods A total of 150 HCC patients were selected for Hepa-tobiliary Surgery in Heping Hospital Affiliated to Changzhi Medical College from January 2018 to May 2020.The expressions of SIRT5 and CRIP1 in HCC tissues and adjacent tissues were detected by immunohistochemistry.The relationship between the expression of SIRT5 and CRIP1 and clinicopathological parameters of HCC patients was analyzed.According to the ex-pression levels of SIRT5 and CRIP1 in HCC tissues,HCC patients were divided into positive/negative SIRT5 and CRIP1 ex-pression groups.Kaplan-Meier method was used to plot the survival curve of HCC patients with positive/negative expres-sion of SIRT5 and CRIP1.The factors influencing the prognosis of HCC patients were analyzed by Coxregression.Results Compared with para-cancerous tissues,HCC tissues showed decreased expression of SIRT5 positivity and increased ex-pression of CRIP1 positivity(χ2 =40.991,42.946,all P＜0.001).HCC tissues with single tumor,small tumor diameter,moderate to high differentiation,no vascular invasion,and TNM stage Ⅰ to Ⅱ,had increased SIRT5 positive expression and de-creased CRIP1 positive expression,all of which were statistically significant(SIRT5:χ2/P=5.179/0.023、6.459/0.011、5.899/0.015、7.402/0.007、5.930/0.015;CRIP1:χ2/P =4.275/0.039、5.442/0.002、6.459/0.011、6.394/0.011、5.655/0.017);At 3-year follow-up,the 3-year overall survival rate of 150 HCC patients was 56.83%(79/139).Kaplan-Meier survival curve analysis showed that the 3-year overall survival rate was higher in the SIRT5-positive expression group than that in the SIRT5-negative ex-pression group,and the 3-year overall survival rate was higher in the CRIP1-positive expression group than that in the CRIP1-negative expression group(Log-rank χ2 = 7.552,20.942,P= 0.006,＜0.001).Multifactorial Cox regression analysis showed that multiple tumor numbers,tumor diameters≥5cm,hypo-differentiation,vascular invasion,TNM stage Ⅲ,and CRIP1 positivity were independent risk factors for death in HCC patients,and SIRT5 positivity was an independent protec-tive factor[OR(95%CI)=2.685(1.031-6.999),2.252(1.143-4.439),4.181(1.735-10.076),3.945(1.653-9.419),3.485(1.492-8.141),4.540(1.641-12.562),0.207(0.085-0.506)].Conclusion Low expression of SIRT5 and high expression of CRIP1 in HCC tissues are associated with tumor number,tumor diameter,degree of differentiation,vascular invasion,TNM stage and prognosis.