The relationship between serum miR-22-3p and NLRP3 levels,inflammatory factors,and poor prognosis in patients with acute cerebral infarction
Ling Xuehui
Xu Wenjie
Qin Yong
Wang Feng
Abstract：Objective To investigate the relationship between serum levels of microribonucleic acid 22-3p(miR-22-3p)and NOD like receptor heat protein domain related protein 3(NLRP3)in patients with acute cerebral infarction(ACI),in-flammatory factors,and poor prognosis.Methods One hundred and six patients with ACI admitted to the Neurology De-partment of the Seventh People's Hospital Affiliated to Shanghai University of Traditional Chinese Medicine from January 2021 to December 2022 were selected as the ACI group.According to the prognosis,they were divided into a subgroup of 37 patients with poor prognosis and a subgroup of 69 patients with good prognosis.Additionally,60 healthy individuals who underwent physical examinations during the same period were selected as the healthy control group.Real time fluores-cence quantitative polymerase chain reaction was used to detect serum miR-22-3p levels.Enzyme-linked immunosorbent as-say for detecting serum NLRP3 and inflammatory factors[interleukin(IL-1)]β,IL-18,Tumor Necrosis Factor-α(TNF-α)].Pearson was used to analyze the relationship between serum miR-22-3p and NLRP3 with IL-1β,IL-18 and TNF-α in ACI patients.Analyze the influencing factors of poor prognosis in ACI patients and draw subject operating characteristic(ROC)curves for two indicators.Results Compared with the healthy control group,the serum levels of miR-22-3p in the ACI group decreased,and the levels of NLRP3,IL-1β,IL-18 and TNF-α increased(t/P= 18.698/<0.001,27.091/<0.001,30.154/<0.001,35.104/<0.001,39.834/<0.001).Pearson correlation analysis showed that serum miR-22-3p was negatively correlated with the levels of NLRP3,IL-1β,IL-18 and TNF-α in ACI patients(r/P=-0.733/<0.001,-0.719/<0.001,-0.683/<0.001,-0.680/<0.001),and serum NLRP3 was positively correlated with the levels of IL-1β,IL-18 and TNF-α(r/P=0.716/<0.001、0.715/<0.001、0.707/<0.001).Multivariate Logistic regression analysis showed that elevated NIHSS score,IL-1β,IL-18,TNF-α,and NLRP3 were independent risk factors for poor prognosis in patients with ACI[OR(95%CI)= 1.244(1.034-1.497),1.373(1.067-1.767),1.047(1.011-1.086),1.577(1.061-2.343),1.084(1.022-1.149)],miR-22-3p was the inde-pendent protection factor[OR(95%CI)=0.933(0.888-0.980)].ROCcurve analysis showed that the area under the curve of serum miR-22-3p and NLRP3 levels combined to predict the poor prognosis of ACI patients was 0.875,which was greater than that predicted by the two alone(0.786 and 0.759(Z/P=2.405/0.016 and 2.517/0.012).Conclusion The decrease of ser-um miR-22-3p level and the increase of NLRP3 level in ACI patients are closely related to the increase of inflammatory factor levels and poor prognosis,and the combined serum levels of miR-22-3p and NLRP3 have a high predictive value for poor prognosis in ACI patients.
Keywords：Cerebral infarctionacuteMicro ribonucleic acid-22-3pNOD-like receptor thermal protein domain asso-ciated protein 3Inflammatory factorPoor prognosis
Publication Date：2024-01-18
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 86-92 )