The predictive value of serum lncRNA LOXL1-AS1 and miR-3614-5p levels for arrhythmia after acute myocardial in-farction

Ma Jiao; Liu Ping; Li Hongmei; Li Chong; Wang Pu; Xiao Yihui

doi:10.3969/j.issn.1671-6450.2024.01.002

Abstract：Objective To investigate the expression levels of long-chain non coding RNA(lncRNA)lysine oxidase like 1-antisense RNA1(LOXL1-AS1)and microRNA-3614-5p in the serum of patients with acute myocardial infarction,as well as their predictive value for arrhythmia.Methods One hundred and forty-eight patients with acute myocardial infarc-tion who were hospitalized in the Department of Cardiology at the First Affiliated Hospital of Xi'an Jiaotong University from January 2021 to January 2023 were selected as the study subjects(acute myocardial infarction group).According to whether the patients developed arrhythmia,they were divided into non arrhythmia subgroup(n=96)and arrhythmia subgroup(n= 52).In addition,148 healthy individuals who matched the general information of acute myocardial infarction patients during the same period were selected as the healthy control group.Compare the levels of serum lncRNA LOXL1-AS1 and miR-3614-5p in each group;Multivariate logistic regression analysis of the influencing factors of arrhythmia after acute myocardi-al infarction;Draw receiver operating characteristic(ROC)curves to analyze the predictive value of serum lncRNA LOXL1-AS1 and miR-3614-5p levels for arrhythmia after acute myocardial infarction.Results Compared with the healthy control group,the level of lncRNA LOXL1-AS1 increased and the level of miR-3614-5p decreased in the acute myocardial infarction group(t/P=16.248/<0.001,8.397/<0.001);The numberofvascularbranches and lncRNA LOXL1-AS1 levels in the arrhyth-mia subgroup were higher than those in the non arrhythmia subgroup,while LVEF and miR-3614-5p levels were lower than those in the non arrhythmia subgroup[χ2(t)/P=14.315/<0.001,7.312/<0.001,3.706/<0.001,7.656/<0.001];The prediction results from Target Scan Human website show that lncRNA LOXL1-AS1 has a binding site with miR-3614-5p,indicating a possible targeting relationship;The results of multivariate logistic regression analysis showed that high lncRNA LOXL1-AS1 and multiple diseased vessel branches were risk factors for arrhythmia after acute myocardial infarction,while high miR-3614-5p and LVEF were protective factors[OR(95%CI)=3.542(1.589-7.896),1.527(1.081-2.156),0.721(0.601-0.865),0.789(0.664-0.938)];The AUC of lncRNA LOXL1-AS1,miR-3614-5p,and their combination in predicting arrhythmia after acute myocardial infarction were 0.820,0.890,and 0.932,respectively.The combination of the two was superior to their individual predictors(Z/P=3.470/0.001,2.293/0.022).Conclusion The serum lncRNA LOXL1-AS1 levels significantly increased and miR-3614-5p levels significantly decreased in patients with arrhythmia after acute myocardial infarction.The combination of the two has good predictive value for arrhythmia after acute myocardial infarction.