Correlation between keratin 7,p53 protein and clinicopathology and prognosis of bladder cancer

Yang Huijuan; Yang Shujun; Wang Yan; Yan Ziqian

doi:10.3969/j.issn.1671-6450.2023.12.015

Abstract：Objective To analyze the correlation between keratin 7(KRT7),p53 protein and clinical pathology and prognosis of bladder cancer.Methods Retrospective analysis was made on the clinical data of 105 patients with bladder cancer admitted to the Pathology Department of the Second People's Hospital of Lianyungang City,Jiangsu Province from February 2016 to March 2019.All patients received radical surgery for bladder cancer.During the surgery,the cancerous tis-sues and adjacent tissues were taken,and the expression levels of KRT7 and p53 proteins in cancerous tissues and adjacent tissues were detected by immunohistochemical method,Cox was used to analyze the prognostic factors of bladder cancer patients,and Kaplan Meier method was used to analyze the relationship between KRT7 protein,p53 protein expression and the prognosis of bladder cancer patients.Results The positive rate of KRT7 protein in cancer tissue is lower than that in adjacent tissues(x2/P=23.786/＜0.001),the positive rate of p53 protein is higher than that of adjacent tissues(x2/P=28.651/＜0.001);Patients with poorly differentiated T2 to T4 stages,tumor diameter ≥ 3 cm,and KRT7 protein positivity in cancer tissue are lower than those with moderately well differentiated Ta to Tl stages,and tumor diameter＜3 cm(x2/P=4.940/0.026,11.299/＜0.001,5.379/0.020),the positive rate of p53 protein is higher than that of patients with Ta-T1 stage,medium to high differentiation,and tumor diameter＜3 cm(x2/P=22.459/＜0.001,12.093/＜0.001,25.527/＜0.001);After 3 years of fol-low-up,4 of 105 bladder cancer patients lost the follow-up,and 34 of the remaining 101 patients died,with a case fatality rate of 33.66％.Cox analysis showed that T2～T4 stage,poor differentiation,tumor diameter ≥ 3 cm,KRT7 protein negative and p53 protein positive were the risk factors for the prognosis of bladder cancer patients[HR(95％CI)=3.935(1.730-8.952),3.575(1.572-8.133),3.515(1 545-7.996),4.933(2.169-11222),3.762(1.654-8.558)].The survival rate of KRT7 protein positive patients is higher than that of KRT7 protein negative patients(x2=5238,P=0.022);The survival rate of p53 pro-tein negative patients is higher than that of p53 protein positive patients(x2=5.115,P=0.024).Conclusion The expression of KRT7 and p53 protein in bladder cancer tissue is closely related to the degree of differentiation,tumor diameter,clinical stage and prognosis.