Expression and significance of serum miR-155 and miR-182 in hemodialysis patients with end-stage renal disease
Zhao Quan
Bi Weijian
Li Pingping
Liu Yang
Yuan Jizhen
Zhu Binwei
Sheng Shujie
Abstract：Objective To investigate the expression and significance ofmicroRNAs miR-155 and miR-182 in serum of hemodialysis patients with end-stage renal disease.Methods A total of 112 patients with end-stage renal disease who received diagnosis and treatment and underwent hemodialysis in the 971st Navy Hospital,Qingdao Municipal Hospital,and Qingdao Haici Hospital from May 2019 to May 2022 were collected as the end-stage renal disease group,and 112 healthy patients who underwent physical examination during the same period were selected as the healthy control group.Real-time quantitative PCR(qRT-PCR)was used to detect the expression of serum miR-155 and miR-182.Pearson method was used to analyze the correlation between serum miR-155 and miR-182 levels in patients with end-end renal disease.Logistic regression analysis was performed to analyze the influencing factors of the prognosis of dialysis patients with end-end renal disease.The receiver operating characteristic(ROC)curve was used to analyze the predictive value of serum miR-155 and miR-182 expression levels in the prognosis of hemodialysis patients with end-stage renal disease.Results The serum expression levels of miR-155 and miR-182 in the end-renal disease group were significantly higher than those in the healthy control group(t/P=12.719/＜0.001,27.901/＜0.001).There were no significant differences in the ratio of males and females,age,BMI,proportion of primary disease,dialysis time,serum phosphorus(P),total cholesterol(TC),triacylglycerol(TG),high-density lipoprotein cholesterol(HDL-C)and low-density lipoprotein cholesterol(LDL-C)between the two subgroups(P＞0.05),and compared with the survival subgroup,the hemoglobin(Hb)and albumin(Alb)of the death subgroup were signifi-cantly reduced(t/P=15.069/＜0.001,9.629/＜0.001),serum calcium(Ca)and high-sensitivity C-reactive protein(hs-CRP)and serum miR-155 and miR-182 were increased(t/P=21.174/＜0.001,40.68/＜0.001,6.629/＜0.001,8.497/＜0.001).The serum ex-pressions of miR-155 and miR-182 in hemodialysis patients with end-end renal disease were positively correlated(r=0.516,P＜0.001).Low Hb,high serum Ca,high hs-CRP,high Alb,high miR-155,and high miR-182 were independent risk factors for the prognosis of dialysis patients with end-renal disease[OR(95％CI)=3.189(1.052-9.670),4.387(1.175-16.375),5.527(1220-25.048),2.058(1251-3386),5561(2.413-12.817),3.189(1.052-9.670)];The area under the curve(AUC)of ser-um miR-155 and miR-182 and their combination were 0.827,0.793 and 0.956,respectively,which were better than those pre-dicted by serum miR-155 and miR-182 separately(Z/P=2.251/0.024,2.797/0.005).Conclusion The serum expression levels of miR-155 and miR-182 in hemodialysis patients with end-stage renal disease are elevated,and the combination of miR-155 and miR-182 may better predict the prognosis of hemodialysis patients with end-stage renal disease.
Keywords：End stage renal diseaseHemodialysisMicroRNA-155MicroRNA-182
Publication Date：2023-12-18
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1292-1296 )