Serum expressions of lncRNA TUG1 and miR-144 in patients with lupus nephritis and their significance

Jiang Dan; Xiong Jinhe; Shang Hua; Wang Jiali; He Fang

doi:10.3969/j.issn.1671-6450.2023.12.010

Abstract：Objective To analyze the relationship between serum long-chain non-coding ribonucleic acid taurine up-regulation gene 1(lncRNA TUG1)and microribonucleic acid-144(miR-144)expression and the prognosis of LN patients with lupus nephritis(LN).Methods A total of 104 LN patients admitted to the Department of Rheumatology and Immunol-ogy of Suining Central Hospital from January 2020 to June 2021 were selected as the LN group,and they were divided into 36 subgroups with poor prognosis and 68 cases with good prognosis according to whether end-stage renal disease(ESRD)occurred during the follow-up period,and 40 healthy patients with physical examination during the same period were select-ed as the healthy control group.The expression of serum lncRNA TUG1 and miR-144 was detected by real-time quantitative polymerase chain reaction kit.The binding sites of lncRNA TUG1 and miR-144 were predicted by TargetScan,and the corre-lation between serum lncRNA TUG1 and miR-144 expression in LN patients was analyzed by Pearson correlation.Multivari-ate logistic regression analysis was used to analyze the risk factors of poor prognosis in LN patients,and receiver operating characteristics(ROC)curves were used to analyze the predictive value of serum lncRNA TUG1 and miR-144 expression in poor prognosis of LN patients.Results Compared with the healthy control group,the expression of serum lncRNA TUG1 in the LN group decreased and the expression of miR-144 increased(t/P=15.885/＜0.001 and 15.808/＜0.001).The Tar-getScan website predicted that there was a binding site between lncRNA TUG1 and miR-144.Pearson correlation analysis showed that serum lncRNA TUG1 was negatively correlated with miR-144 expression in LN patients(r=-0.797,P＜0.001).After 2 years of follow-up,the incidence of ESRD in 104 patients with LN was 34.62％(36/104).Multivariate Logistic regres-sion analysis showed that stage 4 of chronic kidney disease and elevated miR-144 were independent risk factors for poor re-nal prognosis in patients with LN[OR(95％CI)=1.197(1.062～1349),1.108(1.047～1.172)],complete remission after induc-tion therapy and estimated glomerular filtration rate and elevated lncRNA TUG1 were independent protective factors[OR(95％CI)=0305(0.101-0.923),0.979(0.960-0.998),0.841(0.750-0.943)],ROC curve analysis showed that the expression of serum lncRNA TUG1 and miR-144 and the area under the curve(AUC)of the two combined predictions of poor renal prog-nosis in LN patients were 0.772,0.773 and 0.911,respectively,and the AUC predicted by the two was the largest(Z/P=3239/0.001、3247/0.001).Conclusion The low expression of serum lncRNA TUG1 and the high expression of miR-144 in LN patients were associated with poor renal prognosis,and the combined value of the two in predicting the poor renal prognosis of LN was higher.