Factors affecting the transfection efficiency of mouse B lymphoma cell line A20
YANG Cheng
SONG Yuan
YUAN Xiaohan
WANG Huiming
Abstract：Objective To investigate the factors affecting the transfection efficiency of mouse B lymphoma cell line A20 and improve transfection efficiency of suspension cells. Methods The B lymphoma A20 cells were transfected with 4 recombinant plasmids containing a green fluorescent protein ( GFP) reporter gene by nucleofection. Then, the transfection effi-ciency was evaluated by flow cytometry and fluorescence microscopy. The effects of cell transfection time and plasmid size on the efficiency of transfection were assessed. Results The transfection efficiency was high when the plasmid size was below 8KB and the transfection efficiency was highest at 4 hours after transfection. Conclusion Nucleofection is very suitable for transfection of suspension cells with high transfection efficiency. Compared with the lipofection,the nucleofection can effective-ly shorten the transfection time and improve the transfection efficiency.
Keywords：B lymphoma cellCell line A20NucleofectionPlasmid
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 627-629 )