Expression and clinical significance of microRNA-499 in patients with acute myocardial infarction
CAI Xinyu
CHENG Ji
SU Qing
JIANG Li
ZHOU Renjie
Abstract：Objective To study the expression and clinical significance of microRNA-499 in patients with acute myo-cardial infarction. Methods One hundred and seventy-eight cases of acute myocardial infarction who were treated in our hos-pital from February 2015 to December 2016 were selected as the research object. All patients were divided into grade I sub group with 43 cases, grade II sub group with 46 cases,gradeⅢsub group with 45 cases and grade IV sub group with 44 cases according to Killip classification. 50 healthy persons who received medical examination in our hospital in the same period were recorded as control group. The expression of microRNA-499 in all blood samples were detected by real time quantitative PCR method, meanwhile, the serum creatine kinase isoenzyme ( CK-MB) and cardiac troponin I ( cTnI) level were detected by en-zyme linked immunosorbent assay and made correlation analysis, the value of microRNA-499 in early diagnosis of acute myo-cardial infarction was studied by ROC analysis. Results According to the incease of Killip grade, the level of microRNA-499 expression, serum CK-MB and cTnI in the patients were all increased,there were significant differences in the level of blood microRNA-499 expression, serum CK-MB and cTnI levels in the 5 groups (F=1635. 379, F=192. 184, F=321. 842, P=0. 000, P=0. 000, P=0. 000). Pearson correlation analysis showed that there was a positive correlation between microRNA-499 expression and serum CK-MB and cTnI levels in patients with myocardial infarction ( r=0. 584,r=0. 747, P=0. 000, P=0. 000). ROC analysis showed that the level of microRNA-499 expression was of higher value in the early diagnosis of a-cute myocardial infarction, and its' AUC was 0. 839. Conclusion The MicroRNA-499 expression level in patients with acute myocardial infarction show obvious high expression, and it is closely relate to the severity of the disease. In clinical work, the expression of microRNA-499 can be detected to effectively judge the severity of the patients with myocardial infarction, which can be used as one of the biological indicators for early diagnosis of acute myocardial infarction.
Keywords：Acute myocardial infarctionMicroRNA-499Creatine kinase isoenzymeCardiac troponin I
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 541-544 )