Protective effect of Salidroside on the human umbilical vein endothelial cells injury induced by high glucose
LIAO Zhanlin
LIU Pengcheng
ZHANG Lei
LIANG Lifeng
ZHANG Huili
Abstract：Objective To investigate the protective effect of salidroside on the injury of vascular endothelial cells induced by high glucose and possible mechanism.Methods The HUVECs were cultured in vitro.The group of HUVECs that concentration of glucose was 5.5 mmol/L,which were chosen to be the normal control group,and the group of HUVECs that concentration of glucose was 45 mmol/L,which was selected to be the injury model group,then different concentration of Salidroside were added into injury model group,which were 10-5 mol/L,5 × 10-5 mol/L,10-4 mol/L and 2 × 10-4 mol/L.Then cell viability was assessed by MTS assay,the level of intracellular superoxide dismutase (SOD) activity were tested in WST-1,and malondialdehyde (MDA) of the different groups were tested in TBA.Results Compared with the model group,when the HUVECs were cultured in 24 hours and 48 hours,Salidroside concentration of 5x10-5 mol/L,10-4 mol/L and 2x10-4 mol/L had great significance of increasing of the cell viability (F =5.111,P =0.009;F =16.330,P =0.00),the level of intracellular MDA had decreased (F =22.567,P =0.00;F =28.382,P =0.00),and the level of intracellular SOD activity had increased (F =23.388,P =0.00;F =82.244,P =0.00);cultured in 48 hours,compared with the model group,the group that the concentration of Salidroside was 10-5 mol/L showed more significantly increased of the cell viability(P =0.04),and then the level of intracellular MDA activity had increased(P =0.030).Conclusion Salidroside can reduce the level of oxidative stress in HUVEC injury induced by high glucose,thus providing a protective effect on vascular endothelial cells injury induced by high glucose.
Keywords：Diabetic vascular complicationSalidrosideHigh glucoseHuman umbilical vein endothelial cellSuperoxide dismutaseMalondialdehyde
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 494-497,502 )